Volume 2, Number 4 ( (Volume 2 - Issue 4) 2016)                   mazu-cmm 2016, 2(4): 49-53 | Back to browse issues page



DOI: 10.18869/acadpub.cmm.2.4.49

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

sassani E, Khodavaisy S, Agha Kuchak Afshari S, Darabian S, Aala F, Rezaie S. Pseudohyphae formation in Candida glabrata due to CO2 exposure. mazu-cmm. 2016; 2 (4) :49-53
URL: http://cmm.mazums.ac.ir/article-1-140-en.html

Abstract:   (362 Views)

Background and Purpose: Formation of pseudohyphae is considered a virulence factor in Candida species. Generally, Candida glabrata grows as budding yeast cells; however, reports illustrated that C. glabrata could form pseudohyphal cells in response to some stimuli. In this study, we provided insight into the ability of C. glabrata in forming pseudohyphal cells under different levels of carbon dioxide (CO2).
Materials and Methods: Candida glabrata reference strain (ATCC 90030) was used in this study. Yeast samples were cultured on Sabouraud dextrose broth (SDB) medium and incubated under 3%, 5%, and 10% CO2 levels for 24, 48 and72 h. Control cultures were prepared without CO2 pressure for three days. The possibility of pseudohyphae and mycelium formation in C. glabrata was investigated.
Results: The results of this study revealed that the most branching filament-like cells were obtained at high CO2 pressure (10%) after 72 h. After three days of low CO2 pressure (3%), only yeast and budding cells were observed without any pseudohyphae formation.
Conclusion: CO2 could act as a stimulus and induced formation of pseudohyphae in Candida glabrata yeast cells.
 

Full-Text [PDF 634 kb]   (121 Downloads)    
Type of Study: Original Articles | Subject: Medical Mycology
Received: 2017/06/16 | Accepted: 2017/07/31 | Published: 2017/08/28

Add your comments about this article : Your username or email:
Write the security code in the box

Send email to the article author


© 2015 All Rights Reserved | Current Medical Mycology