Volume 3, Issue 1 (March 2017)                   mazu-cmm 2017, 3(1): 30-36 | Back to browse issues page

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Nooshadokht M, Sharifi I, Mohammadi M A, Pirestani M, Afgar A, Mahootchi A, et al . Intestinal Microsporidiosis in Iran (Kerman): Comparison in immune-compromised patients and immune competent people with diarrhea. mazu-cmm. 2017; 3 (1) :30-36
URL: http://cmm.mazums.ac.ir/article-1-147-en.html

Abstract:   (167 Views)
Background and Purpose: Gastroenteritis and the clinical profile caused by Microsporidia, an opportunistic pathogen, may be severe in immunocompromised individuals, especially in AIDS patients. Conventionally, it is necessary to detect the small infective spores in stained smears. However, due to the limitations of the microscopy-based methods, several DNA-based methods such as polymerase chain reaction (PCR) have recently been developed to enhance diagnosis sensitivity. Therefore,we sought to evaluate the rate of infection in immunocompromised patients as compared with immunocompetent patients in Kerman, Iran.
Materials and Methods: We collected stool samples of 199 human subjects (116 males and 83 females), aged 1 to 69 years old. They were divided into immunocompromised (i.e., AIDS [n=72] and cancer-positive patients [n=59]) and immunocompetent (n=68) groups. We comparatively examined the fecal materials using the microscopy and PCR methods.
Results: The overall prevalence rate of Microsporidia infection was 10.05% (20/199). Entrocytozoon bieneusi was the only species within the Microsporidia genus that was identified in 14.5% (19/131) of the immunocompromised patients and 1.47% (1/68) of the immunocompetent individuals.
Conclusion: Considering the higher prevalence rate of microsporidiosis in patients with immunodeficiency (10.03%), we suggest performing sensitive and specific techniques such as PCR for the detection of these parasites in immunocompromised patients.

Full-Text [PDF 386 kb]   (92 Downloads)    
Type of Study: Original Articles | Subject: Medical Mycology
Received: 2017/07/29 | Accepted: 2017/11/6 | Published: 2017/12/13

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