Volume 4, Issue 3 (September 2018)                   Curr Med Mycol 2018, 4(3): 19-22 | Back to browse issues page


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Moazeni M, Hedayati M T, Nabili M. Glabridin triggers over-expression of apoptosis inducing factor (AIF) gene in Candida albicans. Curr Med Mycol. 2018; 4 (3) :19-22
URL: http://cmm.mazums.ac.ir/article-1-203-en.html
Abstract:   (28 Views)
Background and Purpose: Candida albicans is a prevalent human fungal pathogen that can cause a wide spectrum of diseases, from superficial mucosal infections to systemic disorders, in patients with impaired immunity. Glabridin is a pyranoisoflavan originally extracted from root extract of Glycyrrhiza glabra. Glabridin can also mediate apoptosis in yeast cells by changing the mitochondrial membrane potential, activation of caspase-like proteases, and DNA cleavage. The aim of this study was to investigate the mechanism of action of glabridin in C. albicans.
Materials and Methods: Candida albicans ATCC14053 was applied as the standard strain. Total RNA was extracted from the isolate under glabridin-treated and untreated conditions. To evaluate the alternations in the apoptosis inducing factor (AIF) gene expression, real-time polymerase chain reaction (real-time -PCR) was performed, and the obtained data were analyzed using REST software.
Results: Expression of the AIF gene was represented as the ratio of expression relative to the reference gene. According to the REST® output, the expression of the AIF gene increased significantly (P<0.05) under the glabridin-treated condition.
Conclusion: Our results suggested that glabridin may induce apoptosis through the caspase-independent route and might be considered as an anti-Candida agent.
Keywords: AIF gene, Apoptosis, Candida albicans, Glabridin
Full-Text [PDF 164 kb]   (27 Downloads)    
Type of Study: Original Articles | Subject: Medical Mycology
Received: 2018/06/22 | Accepted: 2018/10/9 | Published: 2018/12/5

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