Volume 2, Number 2 (June 2016)                   mazu-cmm 2016, 2(2): 16-19 | Back to browse issues page



DOI: 10.18869/acadpub.cmm.2.2.6
PMID: 28681015
PMCID: PMC5490300

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Arghavan B, Sharifi M, Shafiee M, Mohammadi R. Evaluation of miR-146a expression level in macrophages exposed to Candida glabrata. mazu-cmm. 2016; 2 (2) :16-19
URL: http://cmm.mazums.ac.ir/article-1-98-en.html

Abstract:   (1505 Views)

Background and Purpose: MicroRNAs are small non-coding RNAs with 1924- nucleotides in length. Up- or downregulation
of many miRNAs has been shown by stimulation of Toll-like receptors (TLRs) in the innate immune system.
Up-regulation of miR-146a has been reported by both TLR and heat-killed Candida albicans. In this study, we aimed to
evaluate the expression of miR-146a in cultured monocyte-derived macrophages (MDMs) infected by Candida glabrata
at 12, 24, and 48 hours.
Materials and Methods: miR-146a expression was evaluated by qRT-real time polymerase chain reaction (PCR) at three
time points in C. glabrata-infected MDMs. The data was analyzed using repeated measures ANOVA.
Results: miR-146a expression was down-regulated in infected MDMs compared to the control group (P<0.018). The
expression of miR-146a was at its highest level at 48 h, as compared to 12 and 24 h (P<0.018). The differences between
the experimental group compared to the control group were statistically significant (P<0.018).
Conclusion: These results suggest that miR-146a can be involved in regulating macrophage function following TLR
stimulation in C. glabrata-infected MDMs.

Full-Text [PDF 593 kb]   (526 Downloads)    
Type of Study: Original Articles | Subject: Medical Mycology
Received: 2016/07/24 | Accepted: 2016/09/17 | Published: 2016/10/16

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