The first rare and fatal case of invasive aspergillosis of spinal cord due to Aspergillus nidulans in an Iranian child with chronic granulomatosis disease: review of literature

Document Type : Case report

Authors

1 Student Research Committee, Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Sari, Iran

2 Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Sari, Iran

3 Department of Medical Mycology and Parasitology, Isfahan University of Medical Sciences, Isfahan, Iran

4 Cellular and Molecular Research center, Yasuj University of Medical Sciences, Yasuj, Iran

5 Student Research Committee, Invasive Fungi Research Center, Sari, Iran

6 Department of Clinical pharmacy, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

7 Department of Infectious Diseases, Children Medical Center, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Background and Purpose: Invasive aspergillosis (IA) of the central nervous system (CNS) is a devastating complication which is rarely reported in immunocompromised children.
In this case presentation, we reported a rare and fatal IA with spinal cord involvement in a 10-year-old child with X-linked chronic granulomatosis disease (CGD).
Case report: The child had a previous history of pulmonary tuberculosis. A cervical spine X-ray revealed the involvement of cervical vertebrae (T4/T5) and ribs causing spinal cord compression and epidural abscess. The patient underwent a decompressive laminectomy and mass removal. The histopathology and culture results suggested IA. Despite the aggressive and prolonged therapy, he died within one year. Aspergillus nidulans was identified as the causative agent based on morphological and molecular studies.
Conclusion: This synopsis represents the aggressive behavior of infection caused by A. nidulans in the CGD patient.

Keywords

Introduction

Chronic granulomatous disease (CGD) is a rare inherited disorder of phagocytic cells caused by defects in the nicotinamide adenine dinucleotide phosphate oxidase complex [1]. It may be diagnosed in childhood or adulthood; however, the majority of the affected patients are children under five years of age [2]. These patients may present with few to mild nonspecific clinical symptoms without fever or leukocytosis, even when seriously infected [1].

Although high levels of erythrocyte sedimentation rate (ESR) and serum C-reactive protein (CRP) may be the only indicators, serum CRP is more useful than ESR for the diagnosis and monitoring of infection in CGD patients [3]. The CGD patients may have concurrent bacterial and fungal infections [4]. In an attempt to identify patients with documented bacterial or fungal infections, the medical records of 268 patients with CGD were followed at a single center over 4 decades [4]. In the mentioned study, the incidence of fungal infections was restricted to Aspergillus species, with Aspergillus fumigatus, followed by A. nidulans, accounting for a higher proportion of IA [4].

Aspergillus nidulans is one of the most important and well-known species of the Aspergillus section Nidulantes [5]. Although A. fumigatus has been by far reported as the most common pathogen, A. nidulans is reportedly the most virulent pathogen disseminating to the adjacent bones and then to the brain, thereby resulting in mortality [4]. To date, diverse clinical cases caused by A. nidulans have been reported from patients with otomycosis, mycetoma, keratitis, sinusitis, pulmonary aspergilloma, and primary cutaneous infection associated with a Hickman catheter [6-14]. In addition, the proportion of A. nidulans infections has been reported to be significantly higher in CGD patients with osteomyelitis [15, 16].

In the majority of cases, although CNS aspergillosis involves the brain, the damage to the spinal cord may be a very rare complication in which the spinal cord compression causes vertebral and tissue destruction [17, 18]. Spinal cord infection usually results from hematogenous dissemination from the lung; however, it can rarely develop through contiguous spread [17]. Herein, we report a fatal case of spinal aspergillosis due to A. nidulans following vertebral osteomyelitis in a child suffering from CGD and review all reported cases in the English literature.

Case report

The patient was a 10-year-old boy with confirmed CGD as an underlying disease at 11 months of age based on hematological and immunological tests (nitroblue-tetrazolium=0). He had several admissions to the Children Medical Center of Tehran, Iran. The patient had a previous history of recurrent episodes of pneumonia for which he was treated with broad-spectrum antibiotics and long-term prophylaxis, including itraconazole (5 mg/kg/day), along with co-trimoxazole (5 mg/kg/day) and IFN gamma (1.5 mcg/kg). At the age of nine years, the patient was hospitalized for several clinical signs and symptoms, such as knee pain, night sweets, lethargy, coryza, and progressive weakness.

A plain X-ray imaging showed a soft tissue density destructive lesion in T4-T5 vertebral bodies with adjacent prevertebral soft tissue mass involving the central spinal column. There was also evidence of an expansile lytic lesion involving the right fifth rib, laminectomy of L4-L5 with pedicular screw, along with post-operative changes (Figure 1). Laboratory findings included ESR of 75 mm/h, CRP of 69, white blood cell count of 6000/mm3, monocyte of 37.4%, hemoglobin of 7.8 g/dl, and thrombocyte of 383000/mm3. The results of histopathology and fungal culture obtained from surgical debridement samples revealed the diagnosis of invasive aspergillosis caused by Aspergillus species (Figure 2).

In addition, a microscopic examination showed abundant hull cells and the conidial head pattern of A. nidulans (Figure 2). The specific primer pair 5'-GGT AAC CAA ATC GGT GCT GCT TTC-3' and 5'-ACCCTC AGT GTA GTG ACC CTT GGC-3' was used to amplify β-tubulin genes and confirm the isolate as A. nidulans [19]. Then, the nucleotide sequence was deposited in GenBank under the accession number MK749954. At this time, the treatment was continued with amphotericin B deoxycholate (1 mg/kg/day) which was subsequently changed to voriconazole (9 mg/kg/day). Finally, caspofungin (50 µg/m2) was also added to the therapy, and the patient was discharged from the hospital. However, after a few months, this case was readmitted because of severe headache, blurred vision, and brain abscess, while receiving voriconazole therapy (200 mg/day).

Figure 1.Imaging data in a chronic granulomatosis disease patient with invasive aspergillosis caused by Aspergillus nidulans; a) a plain X-ray revealing the laminectomy of L4-L5 with pedicular screw, along with post-operative changes, and exhibiting a destructive soft tissue density mass lesion centered on the body of T4-T5 vertebrae with adjacent prevertebral soft tissue formation mostly in the right side leading to central spinal column involvement and right fifth rib destruction, b) a computed tomography scan revealing brain abscess leading to hydrocephaly, showing extensive vasogenic edema in the right tempoparietal lobe, along with the dilations of the left ventricle and occipital horn of the right ventricle

Figure 2.Microscopic image of Aspergillus nidulans isolated from the case; a) A. nidulans colonies on Czapek-Dox medium, b) Observation of conidial head structure of A. nidulans stained with Lactophenol Cotton Blue under light microscope (40X magnification), c) smooth round hull cell

Treatment was continued with amphotericin B, caspofungin, voriconazole, and interferon-gamma. Due to the lack of antifungal response and deterioration of the symptoms, he underwent the insertion of a right parietal ventriculoperitoneal shunt. An urgent computed tomography scan of the brain did not depict any damage. However, it revealed the dilation of the left ventricle and dilation of the occipital horn of the right ventricle, along with extensive vasogenic edema in the right temporoparietal lobe (Figure 1).

In spite of performing aggressive surgery and different treatments, the child passed away within one year. Postmortem antifungal susceptibility profile of the isolate was examined against itraconazole (Sigma-Aldrich Química, Madrid, Spain), amphotericin B (Sigma-Aldrich Química, Madrid, Spain), and voriconazole (Pfizer SA, Madrid, Spain) using EUCAST method [20]. The in vitro minimum inhibitory concentrations (MICs) of the mentioned antifungals were obtained as 1, > 16, and 0.25 mg/L, respectively. Furthermore, the minimum effective concentration for caspofungin (Merck & Co., Inc., Rahway, NJ) was estimated at 1 mg/L. This species was found to show resistance to amphotericin B (MIC>2 mg/L).

Discussion

The CGD is a rare disorder that prone the patients to bacterial and opportunistic fungal infections. [3]. Immunodeficiency, broad-spectrum antibiotic use, and Aspergillus colonization of a pre-existing pulmonary cavity may predispose the CGD patients to Aspergillus infection [21]. A review of data from the CGD registry revealed A. fumigatus and A. nidulans as the most common species implicated in the lungs, bones, and brain [16]. The proportion of A. nidulans infections was significantly higher in patients with osteomyelitis, as well as in those receiving itraconazole prophylaxis [16]. Although A. fumigates is the most frequent pathogen isolated from CGD patients, A. nidulans is more virulent and cause a higher fatality rate, compared to A. fumigatus [3].

The present study involved the report of a rare case of spinal aspergillosis following vertebral osteomyelitis in a child with CGD. In this case, a destructive soft tissue density mass lesion was manifested in the spinal column, reflecting the dissemination of infection from an adjacent organ, such as thoracic vertebrae and ribs. Aspergillus vertebral osteomyelitis is an infrequent infection, which can sometimes be complicated by spinal cord compression (Table 1). As indicated in Table 1, curative surgical intervention (laminectomy) is often attempted as a part of patient management for destructive lesions or spinal cord compression.

However, in this case, despite the implementation of aggressive and widespread therapies, brain abscesses were the late presentations of CNS manifestation which were mainly located in the cerebral cortex of the frontal, parietal, and occipital lobes. In a study performed by Van den Berg et al. [18], localized brain abscesses were reported in about 7% of patients with CGD, 38% of which was caused by Aspergillus species.

A literature search was performed using PubMed and Google Scholar databases. The search was accomplished using the following keywords: "Involvement of vertebral bodies" OR "Involvement of spinal cord" OR "CNS infection" AND "A. nidulans" AND "CGD patient". To date, including our case, only 19 relevant cases of vertebral osteomyelitis with or without the involvement of the spinal cord due to A. nidulans have been reported (Table 1). All patients were male with the age range of 2-21 years, with 75% being children and a mortality rate of 47.4%. Vertebral osteomyelitis was reported as the first manifestation in all patients in the presence or absence of pulmonary infection, often leading to the involvement of CNS and spinal cord by contagious dissemination. Therapeutic agents in these patients included liposomal amphotericin B, amphotericin B deoxycholate, flucytosine, itraconazole, and voriconazole, along with adjunctive therapy and aggressive surgery.

Despite advances in early diagnosis and therapeutic approaches, CNS infection due to A. nidulans has still remained as a devastating opportunistic infection, often leading to death. In addition to the high virulence of this species, the use of amphotericin B, as the drug of choice, and the reduced susceptibility of A. nidulans to this antifungal, on the other hand, should be considered additional risk factors [22]. The use of gene therapy for the correction of underlying defects and control of invasive infections due to Aspergillus in CGD patients has also yielded a high success rate [23, 24].

Case Study Age/ Sex Predisposing factor Involved site Surgery Pulmonary infection Prophylaxis Antifungal therapy Adjunctive therapy Brain abscess Outcome
1 Redmond1965 [32] 6/M CGD T1-T8 vertebrae, Ribs Yes Yes No AMB No No Died
2 Bujak etal., 1974 [33] 8/M CGD Fourth rib No Yes Clindamycin AMB Leukocyte transfusion No Survived
3 Altman etal., 1977 [34] 10/M CGD 2nd–3rd ribs, Vertebrae Yes Yes No AMB No No Survived
4 White 1987 [35] 4/M CGD T8-10 Vertebrae, 7th ribs, Cord compression Yes Yes No AMB flucytosine, Itraconazole Granulocyte transfusion No Survived
5 Sponselleret al., 1991[36] 4/M CGD 8th-9th ribs, T6-L1 vertebrae Yes No NA AMB, imipenem Granulocyte transfusion No Died
6 Sponselleret al., 1991 [36] 9/M CGD 4th rib Yes Yes NA AMB No No Survived
7 Sponseller et al., 1991 [36] 13/M CGD T3-T4 vertebrae, 4th rib Yes No NA AMB No No Survived
8 Kim et al., 1997 [37] 6/M CGD T5-T6 vertebrae Yes Yes TMP-SMX AMB, ITZ, IFN-α, GM-CSF No Died
9 Ozsashin et al., 1998 [38] 8/M CGD 6th rib, Yes Yes Itraconazole, TMP-SXT Deoxycholate AMB, LAMB IFNγ, G-CSF No Survived (BM transplantation)
10 Segal et al., 1998 [7] 19/M CGD 3rd rib, T3 Vertebrae, Spinal cord compression, temporal fossa involvement Yes Yes No AMB, itraconazole, flucytosine IFNγ, Leukocyte transfusion, The instillation of granulocytes and hydrogen peroxide Yes Died
11 Segal et al., 1998 [7] 16/M CGD 3rd-4th rib, T4 vertebrae Yes Yes No Deoxycholate AMB, ketoconazole, itraconazole, liposomal AMB IFNγ, leukocyte transfusion No Died
12 Segal et al., 1998 [7] 4/M CGD Vertebrae, Cord compression Yes Yes NA AMB Leukocyte transfusion NA Died
13 Segal et al., 1998 [7] 7/M CGD Ribs, paraspinal mass, the involvement of sinuses, nasal cavity and orbits Yes Yes No Deoxycholate AMB, LAMB IFNγ, leukocyte transfusion Yes Died
14 Notheis et al., 2006 [23] 2/M CGD T2-T5 vertebrae, infiltration of spinal cord Yes Yes TMP-SXT, Itraconazole Deoxycholate AMB, voriconazole and caspofungin, posaconazole, LAMB, leukocyte transfusion No Survived (ex-vivo gene therapy)
15 Sastry et al., 2006 [39] 18/M CGD Rib, Vertebrae Yes Yes TMP-SMX, Itraconazole Liposomal AMB, voriconazole and caspofungin No Yes Survived (BM transplantation)
16 Dellapianeet al., 2008 [40] 21/M CGD T5-T7 vertebrae, SPINAL cord compression, ribs Yes Yes TMP-SMX, ITZ Liposomal AMB, caspofungin, voriconazole IFNγ probably Died
17 Bukhari etal., 2009 [41] 5/M CGD T1, T2, T4 and T6 vertebrae, Spinal cord compression Yes Yes NA Voriconazole No No Survived
18 Khalid2018 [42] 9/M CGD 3rd rib No Yes TMP-SMX, ITZ Voriconazole No No Survived
19 PresentCase 10/M CGD T4-T5 vertebrae, fifth rib, spinal cord compression Yes Yes ATT, Co-trimoxazole, Itraconazole AMB, voriconazole and caspofungin IFNγ Yes Died
CGD: chronic granulomatosis disease, GM-CSF: granulocyte macrophage stimulating factor, TMP-SMX: trimethoprim-sulfamethoxazole, NA: non-available, ATT: anti-tuberculosis drugs, BMT: bone marrow transplantation
Table 1.Demographic characteristics, clinical data, and treatment profiles of chronic granulomatosis disease patients affected with vertebral osteomyelitis and central nervous system aspergillosis due to Aspergillus nidulans

Briefly, our study highlights some important points. Firstly, the elevated levels of non-specific markers, such as
ESR and CRP, may be helpful for the diagnosis and monitoring of vertebral osteomyelitis [25]. Nevertheless, significantly elevated levels of ESR and CRP are more valuable markers for the establishment of presumptive diagnosis and evaluation of patients by specific tests, such as galactomannan for IA [26]. Galactomannan as a predictive index could lead to the early diagnosis and rapid treatment of osteomyelitis due to aspergillosis [27]. Secondly, itraconazole is typically used for prophylaxis against IA. However, a long-term use of itraconazole can be a possible risk factor for developing IA due to A. nidulans in CGD patients [16].

Thirdly, in CGD patients suffering from IA, IFN-γ is crucial for both Th1-dependent protective immunity and phagocytic cell function against fungi [28]. Regarding this, IFN-γ was administered as adjunctive therapy in combination with voriconazole or amphotericin B in our patient, and a 10-fold increase of monocyte count was found without a significant difference in survival rate.

Extracellular killing or oxidase-dependent NETosis induction is a possible reason for the difference of antimicrobial activity of CGD PMNs against A. nidulans, compared with A. fumigates [29]. Accordingly, despite the administration of higher doses of IFN-γ to CGD patients with A. nidulans osteomyelitis, survival rate remains no change. Moreover, in this patient, amphotericin B was considered first-line therapy; however, refractory to this antifungal was observed which can be the result of reduced susceptibility to amphotericin B [7]. Voriconazole has also shown higher response and improved survival rates than amphotericin B. This antifungal has been recommended as the preferred agent for the treatment of IA [30]. In addition, in clinical series, combination therapy with voriconazole and echinocandins has been employed to increase the efficacy of voriconazole monotherapy [31].

The present study involved the evaluation of the in vitro antifungal activity of only one isolate of A. nidulans. Therefore, the obtained results may not reflect the actual estimation of the clinical co-administration of voriconazole and echinocandins.

Conclusion

The determination of MIC based on the reference procedures is a gold standard to detect resistant clinical isolates and consider the best antifungal therapy to improve patient outcomes. Moreover, voriconazole is significantly more effective for the treatment of IA due to A. nidulans in the CGD patients, compared to amphotericin B deoxycholate; therefore, it should be preferred.

References

  1. Almyroudis NG, Holland SM, Segal BH. Invasive aspergillosis in primary immunodeficiencies. Med Mycol. 2005; 43(Suppl 1):S247-59.
  2. Martire B, Rondelli R, Soresina A, Pignata C, Broccoletti T, Finocchi A. Clinical features, long-term follow-up and outcome of a large cohort of patients with chronic granulomatous disease: an Italian multicenter study. Clin Immunol. 2008; 126(2):155-64.
  3. Segal BH. Invasive aspergillosis in chronic granulomatous disease Aspergillosis: from diagnosis to prevention. Springer: New York; 2009.
  4. Marciano BE, Spalding C, Fitzgerald A, Mann D, Brown T, Osgood S. Common severe infections in chronic granulomatous disease. Clin Infect Dis. 2014; 60(8):1176-83.
  5. Chen AJ, Frisvad JC, Sun BD, Varga J, Kocsubé S, Dijksterhuis J. Aspergillus section Nidulantes (formerly Emericella): polyphasic taxonomy, chemistry and biology. Stud Mycol.. 2016; 84:1-118.
  6. Yu J, Mu X, Li R. Invasive pulmonary aspergillosis due to Emericella nidulans var echinulata successfully cured by voriconazole and micafungin. J Clin Microbiol. 2013; 51(4):1327-9.
  7. Segal BH, DeCarlo ES, Kwon-Chung KJ, Malech HL, Gallin JI, Holland SM. Aspergillus nidulans infection in chronic granulomatous disease. Medicine. 1998; 77(5):345-54.
  8. Verweij PE, Varga J, Houbraken J, Rijs AJ, VerduynLunel FM, Blijlevens NM. Emericella quadrilineata as cause of invasive aspergillosis. Emerg Infect Dis. 2008; 14(4):566-72.
  9. Baylet R, Basset A. Aspergillus nidulans, agent of mycetoma. Bull Soc Pathol Exot Filiales. 1968; 61(3):359-65.
  10. Doby JM, Kombila-Favry M. Presence of sexual forms (cleistothecia and Hülle cells) in a case of human maxillary sinus aspergillosis caused by Aspergillus nidulans in association with Aspergillus fumigatus. Mycopathologia. 1978; 64(3):157-63.
  11. Joshi KR, Mathur DR, Sharma JC, Vyas MC, Sanghvi A. Mycetoma caused by Aspergillus nidulans in India. J Trop Med Hyg. 1985; 88(1):41-4.
  12. Henriet SS, Verweij PE, Warris A. Aspergillus nidulans and chronic granulomatous disease: a unique host–pathogen interaction. J Infect Dis. 2012; 206(7):1128-37.
  13. Sabino R, Veríssimo C, Parada H, Brandão J, Viegas C, Carolino E. Molecular screening of 246 Portuguese Aspergillus isolates among different clinical and environmental sources. Med Mycol. 2014; 52(5):519-29.
  14. Lucas GM, Tucker P, Merz WG. Primary cutaneous Aspergillus nidulans infection associated with a Hickman catheter in a patient with neutropenia. Clin Infect Dis. 1999; 29(6):1594-6.
  15. Dotis J, Roilides E. Osteomyelitis due to Aspergillus spp in patients with chronic granulomatous disease: comparison of Aspergillus nidulans and Aspergillus fumigatus. Int J Infect Dis. 2004; 8(2):103-10.
  16. Blumental S, Mouy R, Mahlaoui N, Bougnoux ME, Debré M, Beauté J. Invasive mold infections in chronic granulomatous disease: a 25-year retrospective survey. Clin Infect Dis. 2011; 53(12):e159-69.
  17. Sheth NK, Varkey B, Wagner DK. Spinal cord aspergillus invasion--complication of an aspergilloma. Am J Clin Pathol. 1985; 84(6):763-9.
  18. van den Berg JM, van Koppen E, Åhlin A, Belohradsky BH, Bernatowska E, Corbeel L. Chronic granulomatous disease: the European experience. PloS One. 2009; 4(4):e5234.
  19. Nouripour-Sisakht S, Mirhendi H, Shidfar M, Ahmadi B, Rezaei-Matehkolaei A, Geramishoar M. Aspergillus species as emerging causative agents of onychomycosis. J Mycol Med. 2015; 25(2):101-7.
  20. Arendrup MC, Guinea J, Cuenca-Estrella M, Meletiadis J, Mouton JW, Lagrou K. Eucast Definitive Document E. DEF 9.3. Method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for conidia forming moulds. European Committee for Antimicrobial Susceptibility Testing: Vaxjo, Sweden; 2015.
  21. Liese J, Kloos S, Jendrossek V, Petropoulou T, Wintergerst U, Notheis G. Long-term follow-up and outcome of 39 patients with chronic granulomatous disease. J Pediatr. 2000; 137(5):687-93.
  22. Horwitz ME, Barrett AJ, Brown MR, Carter CS, Childs R, Gallin JI. Treatment of chronic granulomatous disease with nonmyeloablative conditioning and a T-cell-depleted hematopoietic allograft. N Engl J Med. 2001; 344(12):881-8.
  23. Notheis G, Tarani L, Costantino F, Jansson A, Rosenecker J, Friederici D. Posaconazole for treatment of refractory invasive fungal disease. Mycoses. 2006; 49(Suppl 1):37-41.
  24. Malech HL. Progress in gene therapy for chronic granulomatous disease. J Infect Dis. 1999; 179(Suppl 2):S318-25.
  25. Tsiodras S, Falagas ME. Clinical assessment and medical treatment of spine infections. Clin Orthop Relat Res.. 2006; 444:38-50.
  26. Sambatakou H, Guiver M, Denning D. Pulmonary aspergillosis in a patient with chronic granulomatous disease: confirmation by polymerase chain reaction and serological tests, and successful treatment with voriconazole. Eur J Clin Microbiol Infect Dis. 2003; 22(11):681-5.
  27. Gamaletsou MN, Rammaert B, Bueno MA, Moriyama B, Sipsas NV, Kontoyiannis DP. Aspergillus osteomyelitis: epidemiology, clinical manifestations, management, and outcome. J Infect. 2014; 68(5):478-93.
  28. Mamishi S, Zomorodian K, Saadat F, Gerami-Shoar M, Tarazooie B, Siadati SA. A case of invasive aspergillosis in CGD patient successfully treated with Amphotericin B and INF-γ. Ann Clin Microbiol Antimicrob. 2005; 4(1):4.
  29. Henriet SS, Hermans PW, Verweij PE, Simonetti E, Holland SM, Sugui JA. Human leukocytes kill Aspergillus nidulans by reactive oxygen species-independent mechanisms. Infect Immun. 2011; 79(2):767-73.
  30. Herbrecht R, Denning DW, Patterson TF, Bennett JE, Greene RE, Oestmann JW. Voriconazole versus amphotericin B for primary therapy of invasive aspergillosis. N Engl J Med. 2002; 347(6):408-15.
  31. Siopi M, Siafakas N, Vourli S, Mouton JW, Zerva L, Meletiadis J. Dose optimization of voriconazole/ anidulafungin combination against Aspergillus fumigatus using an in vitro pharmacokinetic/pharmacodynamic model and response surface analysis: clinical implications for azole-resistant aspergillosis. J Antimicrob Chemother. 2016; 71(11):3135-47.
  32. Redmond A, Carre IJ, Biggart JD, Mackenzie DW. Aspergillosis (Aspergillus nidulans) involving bone. J Pathol Bacteriol. 1965; 89(1):391-5.
  33. Bujak JS, Kwon-Chung KJ, Chusid MJ. Osteomyelitis and pneumonia in a boy with chronic granulomatous disease of childhood caused by a mutant strain of Aspergillus nidulans. Am J Clin Pathol. 1974; 61(3):361-7.
  34. Altman A. Thoracic wall invasion secondary to pulmonary aspergillosis: a complication of chronic granulomatous disease of childhood. Am J Roentgenol. 1977; 129(1):140-2.
  35. White CJ, Kwon-Chung K, Gallin JI. Chronic granulomatous disease of childhood: an unusual case of infection with Aspergillus nidulans var echinulatus. Am J Clin Pathol. 1988; 90(3):312-6.
  36. Sponseller PD, Malech HL, McCarthy EF Jr, Horowitz SF, Jaffe G, Gallin JI. Skeletal involvement in children who have chronic granulomatous disease. J Bone Joint Surg Am. 1991; 73(1):37-51.
  37. Kim M, Shin JH, Suh SP, Ryang DW, Park CS, Kim CS. Aspergillus nidulans infection in a patient with chronic granulomatous disease. J Korean Med Sci. 1997; 12(3):244-8.
  38. Ozsahin H, Von Planta M, Müller I, Steinert HC, Nadal D, Lauener R. Successful treatment of invasive aspergillosis in chronic granulomatous disease by bone marrow transplantation, granulocyte colony-stimulating factor–mobilized granulocytes, and liposomal amphotericin-B. Blood. 1998; 92(8):2719-24.
  39. Sastry J, Kakakios A, Tugwell H, Shaw PJ. Allogeneic bone marrow transplantation with reduced intensity conditioning for chronic granulomatous disease complicated by invasive Aspergillus infection. Pediatr Blood Cancer. 2006; 47(3):327-9.
  40. Dellepiane RM, Tortorano AM, Liotto N, Laicini E, Di Landro G, Carnelli V. Invasive Aspergillus nidulans infection in a patient with chronic granulomatous disease. Mycoses. 2008; 51(5):458-60.
  41. Bukhari E, Alrabiaah A. First case of extensive spinal cord infection with Aspergillus nidulans in a child with chronic granulomatous disease. J Infect Dev Ctries. 2009; 3(4):321-3.
  42. Khalid M, Ali SA. Fungal osteomyelitis in a patient with chronic granulomatous disease: Case report and review of the literature. J Pak Med Assoc. 2018; 68(9):1387-90.
Current Medical Mycology
Volume 6, Issue 1
March 2020
Pages 55-60

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