Isolation of different fungi from the skin of patients with seborrheic dermatitis

Document Type: Short Communication

Authors

1 Department of Mycology, School Of Medicine, Alborz University of Medical Sciences, Karaj, Iran.

2 Department of Medical Laboratory Sciences, School of Paramedicine, Alborz University of Medical Sciences, Karaj, Iran

Abstract

Background and Purpose: Seborrheic dermatitis (SD) is characterized by erythematous inflammatory patches that mostly appear in the sebaceous gland-rich skin areas. In addition to the key role of Malassezia species in SD, its contribution to other fungal microbiota has been recently addressed in the literature. Regarding this, the present study was conducted to identify and determine the fungal species associated with the incidence of SD.
Materials and Methods: For the purpose of the study, fungal microbiome in scaling samples were collected from SD lesions and then analyzed based on the DNA sequencing of ITS regions.
Results: In addition to Malassezia, several fungal species were detected in the samples collected from the SD lesions. According to the results, 15.5%, 13.3%, and 6.7% of the isolates were identified as Candida parapsilosis, Cryptococcus albidus var. albidus/ Rhodotorula mucilaginosa, and Penicillium polonicum, respectively.
Conclusion: Based on the obtained results, C. parapsilosis was the most prevalent non-Malassezia species isolated from SD lesions. Our results provided basic information about a specific fungal population accounting for the incidence of SD.

Keywords


1. Byrd AL, Belkaid Y, Segre JA. The human skin microbiome. Nat Rev Microbiol. 2018; 16(3):143-55.
2. Underhill DM, Iliev ID. The mycobiota: interactions between commensal fungi and the host immune system. Nat Rev Immun.
2014; 14(6):405-16.
3. Zhang E, Tanaka T, Tajima M, Tsuboi R, Nishikawa A, Sugita T. Characterization of the skin fungal microbiota in patients with atopic dermatitis and in healthy subjects. Microbiol Immunol. 2011; 55(9):625-32.
4. Niemeyervan der Kolk T, van der Wall HE, Balmforth C, Van Doorn MB, Rissmann R. A systematic literature review of the human skin microbiome as biomarker for dermatological drug development. Br J Clin Pharmacol. 2018; 84(10):2178-93.
5. Rodrigues Hoffmann A. The cutaneous ecosystem: the roles of the skin microbiome in health and its association with inflammatory skin conditions in humans and animals. Vet Dermatol. 2017; 28(1):60-e15.
6. Findley K, Grice EA. The skin microbiome: a focus on pathogens and their association with skin disease. PLoS Pathog. 2014; 10(10):e1004436.
7. Theelen B, Cafarchia C, Gaitanis G, Bassukas ID, Boekhout T, Dawson TL Jr. Malassezia ecology, pathophysiology, and treatment. Med Mycol. 2018; 56(Suppl 1):S10-25.
8. Hedayati M, Hajheydari Z, Hajjar F, Ehsani A, Shokohi T, Mohammadpour R. Identification of Malassezia species isolated from Iranian seborrhoeic dermatitis patients. Eur Rev Med Pharmacol Sci. 2010; 14(1):63-8.
9. Prohic A, Jovovic Sadikovic T, Krupalija-Fazlic M, Kuskunovic-Vlahovljak S. Malassezia species in healthy skin and in dermatological conditions. Int J Dermatol. 2016; 55(5):494-504.
10. Zoulba EN, Widaty S, Krisanty IA, Wahid MH. Identification of Malassezia species and the severity of seborrheic dermatitis on scalp in Dr. Cipto Mangunkusumo Hospital Jakarta. Adv Sci Let. 2018; 24(9):6649-52.
11. Faergemann J. Management of seborrheic dermatitis and pityriasis versicolor. Am J Clin Dermato. 2000; 1(2):75-80.
12. Bumroongthai K, Chetanachan P, Niyomtham W, Yurayart C, Prapasarakul N. Biofilm production and antifungal susceptibility of co-cultured Malassezia pachydermatis and Candida parapsilosis isolated from canine seborrheic dermatitis. Med Mycol. 2016; 54(5):544-9.
13. Chowdhry S, Gupta S, D’souza P. Topical antifungals used for treatment of seborrheic dermatitis. J Bacteriol Mycol Open Access. 2017; 4(1):76.
14. Mahmoudi E, Saeidi M, Marashi MA, Moafi A, Mahmoodi V, Zeinolabedini Zamani M. In vitro activity of kombucha tea ethyl acetate fraction against Malassezia species isolated from seborrhoeic dermatitis. Curr Med Mycol. 2016; 2(4):30-6.
15. Kalantar E, Marashi SM, Pormazaheri H, Mahmoudi E, Hatami S, Barari MA, et al. First experience of Candida non-albicans isolates with high antibiotic resistance pattern caused oropharyngeal candidiasis among cancer patients. J Can Res Ther. 2015; 11(2):388-90.
16. Yamada Y, Makimura K, Merhendi H, Ueda K, Nishiyama Y, Yamaguchi H, et al. Comparison of different methods for extraction of mitochondrial DNA from human pathogenic yeasts. Jpn J Infect Dis. 2002; 55(4):122-5.
17. Gupta AK, Boekhout T, Theelen B, Summerbell R, Batra R. Identification and typing of Malassezia species by amplified fragment length polymorphism and sequence analyses of the internal transcribed spacer and large-subunit regions of ribosomal DNA. J Clin Microbiol. 2004; 42(9):4253-60.
18. Trofa D, Gacser A, Nosanchuk JD. Candida parapsilosis, an emerging fungal pathogen. Clin Microbiol Rev. 2008; 21(4):606-25.
19. Kohler JR, Casadevall A, Perfect J. The spectrum of fungi that infects humans. Cold Spring Harb Perspect Med. 2015; 5(1):a019273.
20. Yin W, Wang Y, Liu L, He J. Biofilms: the microbial “protective clothing” in extreme environments. Int J Mol Sci. 2019; 20(14):E3423.