Influence of ascorbic acid and α-tocopherol on the autoxidation and in vitro antifungal activity of amphotericin B

Document Type : Original Articles


1 Université de Ghardaia, Department of Biology, BP455, Ghardaia 47000, Algeria

2 Antifungal Antibiotics Laboratory: Physical Chemistry, Synthesis, and Biological Activity, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe Sciences, University of Tlemcen, Tlemcen, Algeria


Background and Objectives: Amphotericin B (AmB) isthe standard treatment for systemic fungal infections; however, the formation of reactive oxygen species reducesthe efficacy and stability of this molecule. The presentstudy aimed to evaluate the effect of the combinationof AmB with ascorbic acid and α-tocopherolon itsautoxidation andantifungal activity.
Materials and Methods: The antifungal activity against Candida albicans was evaluated bythe viable cell counting methodandchecking their morphological changes with a scanning electron microscope.Monomer state of AmB wasassessedby scanning the UV absorbance in the range of 300-450 nm and the lipid peroxidation was measured using quantification of thiobarbituric acid reactive-substances (TBARS).
Results:Based on the findings, the addition of ascorbic acid (3×102 µg/mL) and α-tocopherol (16 µg/mL) to the reaction medium of AmBincreasedits antifungal activity while maintaining itsmolecular stability. Moreover, the level of TBARS formed in the reaction medium of AmB was significantly reduced after combination with ascorbic acid and α-tocopherol.
Conclusion: Given their availability, their anti-free radical activity, and their low toxicity, the incorporation of ascorbic acid and α-tocopherol into the reaction medium of AmB seems to be a promising approach to obtain an effective antifungal formulation.