Effects of Bunium persicum essential oil on the reduction of spore germination, growth, and expression of FUM1 and FUM14 genes in Fusarium verticillioides isolates

Document Type : Original Articles

Authors

1 Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranUniversity of Tehran

2 Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran

10.18502/CMM.7.2.7033

Abstract

Background and Purpose: Bunium persicum (Zire Kermani) is an plant which is commonly used as an antispasmodic, carminative and antimicrobial substance. This investigation was carried out to assess different components of B. persicum essential oil (EO) and its effects on fungal growth, spore germination and FUM1 and FUM14 genes expression in Fusarium verticillioides.
Materials and Methods: The plant EO was extracted by hydrodistillation and analyzed by using gas chromatography- mass spectroscopy (GC-MS). A broth microdilution method was used for determining the minimum inhibitory concentration (MIC) of the plant EO. In addition, the expression of FUM1 and FUM14 genes of toxigenic F. verticillioides was assessed by using real time-PCR (RT-PCR).
Results: γ-terpinene (15.56%) and propanal, 2-methyl-3-phenyl (14.18%) were the main components of the plant EO. The EO showed a good antifungal activity (mean MIC value: 2556.8 μg/ml) as well as the inhibition of spore germination and mycelial growth (p<0.05). RT-PCR demonstrated that the mean expression levels of FUM1 and FUM14 of B. persicum -treated F. verticillioides were 0.43 and 0.53 folds lower than the control samples, respectively (p<0.05).
Conclusion: The results showed that B. persicum EO can be used to reduce the mycelial growth and spore germination and also reduce the expression of genes involved in the production of fumonisin by F. verticillioides.

Keywords