Document Type : Original Articles
Department of Medical Parasitology and Mycology, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran
Mazandaran university of medical sciences
University of Medical Sciences, Bushehr, Iran
General Medical Education and Research Center, Teikyo University, Tokyo, Japan
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
Department of Environmental Health Engineering, School of Public Health, Isfahan University of Medical Sciences, Isfahan, Iran
5) Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
National Institute of Health Research Isfahan Health Research Station, Tehran University of Medical Sciences, Tehran, Iran
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Medical Parasitology &amp; Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Laboratory of Space and Environmental Medicine, Graduate School of Medicine, Teikyo University, Tokyo, Japan. 5 General Medical Education and Research Center, Teikyo University, Tokyo, Japan
Background and Purpose: The frequency and genetic diversity of black fungi in environmental and clinical settings remains to be performed in Iran. This study aim to identify and evaluate intra- and inter-species DNA sequence variation, in addition to understand phylogenetic relationships of melanized fungi and relatives isolated from different geographical regions of Iran.
Materials and Methods: One hundred and eleven clinical and environmental strains of dematiaceous fungi were isolated, and their ITS rDNA region were sequenced and analyzed.
Results: An inter-species nucleotide sequence diversity rate of one to 464 nucleotides was observed between the species. Intra-species differences were found in the strains of Alternaria alternata, Cladosporium cladosporioides, Alternaria tenuissima, Curvularia spicifera, Aureobasidium pullulans, Curvularia hawaiiensis, Neoscytalidium dimidiatum, Alternaria terricola, Alternaria chlamydospora, Didymella glomerata and Drechslera dematioidea by 0–59, 0–22, 0–4, 0–4, 0–3, 0–2, 0–2, 0–2, 0–2, 0–1 and 0–1 nt, respectively.
Conclusions: ITS rDNA is useful for discrimination of several taxa of dematiaceous fungi; for species of Alternaria, however, a larger rDNA region or a library of other gene sequences is essential for better understanding of the taxonomy of this group of fungi.