Molecular identification of Candida species isolated from candiduria and its risk factors in neonates and children

Document Type : Original Articles


1 Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2 Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran



Background and Purpose: The present study was performed to raise attention on the
frequency of Candida spp. and evaluation of risk factors of candiduria in neonates and
Materials and Methods: In total, 60 urine samples were collected from the suspected
neonates and children. Identification of Candida at species level was performed using
the polymerase chain reaction-restriction fragment length polymorphism approach.
Results: The restriction fragment length polymorphism fingerprint analysis revealed
that Candida parapsilosis (n=17; 28.33 %) is the most prevalent isolated species
followed by Candida albicans (n=9; 15%), Candida tropicalis (n=4; 9.52%), and C.
glabrata (n=2; 4.76%). All of the C. albicans and C. parapsilosis complex strains were
identified as C. albicans with HWP1 gene primers and using the NlaIII restriction
enzyme activity, respectively. In this study, none of the mentioned factors was the cause
of infection, but they could be considered risk factors. The mean hospital stay was 21
days (range: 7-21 days). More than 90% of the patients had a urinary catheter, and about
26% of them received antibiotics. Regarding the risk factors, there was no significant
difference between the two groups of candidiasis in terms of C. albicans and non
albicans Candida (P<0.01).
Conclusion: Candiduria has always been a challenging issue, especially in children
admitted to hospitals. Outcome of candiduria in patients with generally healthy is little.