Molecular identification of Candida species isolated from candiduria and its risk factors in neonates and children

Document Type : Original Articles

Authors

1 Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2 Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Objective: The present study was performed to raise attention on frequency of Candida spp. and evaluation of risk factors of candiduria in pediatric.
Methods: 60 urine samples were collected from the suspected pediatric. Identification of Candida at species level was done using the PCR- RFLP approach.
Results: RFLP fingerprint analysis revealed that Candida parapsilosis (n:17; 28.33 %) known as the most prevalent isolated species followed by Candida albicans (n: 9; 15%), Candida tropicalis (n: 4; 9.52%), C. glabrata (n: 2; 4.76%). All of the C. albicans and C. parapsilosis complex strains were identified as C. albicans with HWP1 gene primers and using the NlaIII restriction enzyme activity, respectively. In this study, None of the mentioned factors is cause of infection, they could be considered as risk factors. The average hospital stay was 21 days, at least 7 and 21 days. more than 90% of patients had urinary catheter, and about 26% of the pediatric received antibiotics. Regarding the risk factors, there was no significant difference between the two groups of candidiasis by C. albicans and non- albicans Candida (NAC) (P< 0.01).
Conclusion: Candiduria have always been a challenging issue, especially in children admitted to hospitals. Outcome of candiduria in patients with generally healthy is little.
 

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