Chemical Composition and Antifungal Activities of Aromatic Water of Zataria Multiflora Boiss

Document Type : Original Articles

Authors

1 Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz, Iran

2 Department of Medical Mycology and Parasitology, Shiraz University of Medical Sciences, Shiraz, Iran

3 Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

4 Molecular Dermatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

5 Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

6 Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

10.18502/cmm.7.3.7255

Abstract

Background and Purpose: In Iranian culture, aromatic waters harboring slight amount of essential oil has been popularly used for many years as a pleasant non-alcoholic drink with various medicinal properties.
In this study chemical composition of Zataria multiflora Boiss aromatic water was determined and it’s in vitro and in vivo antifungal properties were investigated.
Material andMethods: Chemical composition of the essential oil extracted (EO) from aromatic water (AW) of Z. multiflora was analyzed by Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The antimicrobial activity of the AW against Candida species was determined by broth micro-dilution methods. Additionally biofilm formation inhibition and antioxidant activity of the AW were measured using a XTT reduction and DPPH methods, respectively. Anitifungal activity of the AW in the prevention and/or treatment of gastrointestinal (GI) candidiasis were also evaluated in animal model.
Results: GC-MS analysis revealed that the major constituents of Z. multiflora AW were Carvacrol (46.56%) and Thymol (40.67%). The Z. multiflora AW inhibited the growth and biofilm formation of Candida species in the range of 0.25-0.5 V/V. Moreover, Z. multiflora AW significantly decreased the Candida colonization in therapeutic groups (P < 0.05).
Conclusion: Given the wide therapeutic potential of Z. multiflora AW including antifungal and antioxidant activities, it might be possible to be used in the management of mucocutaneous or alimentary candidiasis.

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