Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Medical Genetics, School of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran
Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Background and Purpose: MicroRNAs are small non-coding RNAs with 1924- nucleotides in length. Up- or downregulation
of many miRNAs has been shown by stimulation of Toll-like receptors (TLRs) in the innate immune system.
Up-regulation of miR-146a has been reported by both TLR and heat-killed Candida albicans. In this study, we aimed to
evaluate the expression of miR-146a in cultured monocyte-derived macrophages (MDMs) infected by Candida glabrata
at 12, 24, and 48 hours.
Materials and Methods: miR-146a expression was evaluated by qRT-real time polymerase chain reaction (PCR) at three
time points in C. glabrata-infected MDMs. The data was analyzed using repeated measures ANOVA.
Results: miR-146a expression was down-regulated in infected MDMs compared to the control group (p <0.018). The
expression of miR-146a was at its highest level at 48 h, as compared to 12 and 24 h (p <0.018). The differences between
the experimental group compared to the control group were statistically significant (p <0.018).
Conclusion: These results suggest that miR-146a can be involved in regulating macrophage function following TLR
stimulation in C. glabrata-infected MDMs.