Department of Medical Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Department of Prosthodontics, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Background and Purpose: In people wearing dentures, the growth of various Candida species under the prosthesis
leads to the formation of biofilm, which can play the role of a reservoir for Candida and other kinds of microbes. Since
nano-chitosan particles can cause lasting antimicrobial activity, a more recent approach that utilizes acrylic resins with
nano-chitosan particles is proposed. Therefore, we aimed to study the inhibitory effect of nano-chitosan particles on the
biofilm formation of Candida species in acrylic resins.
Materials and Methods: In this analytical in-vitro study, acrylic resins with nano-chitosan particles with concentrations of
0, 1%, 5%, and 10% were put adjacent to the suspension of Candida cells isolated from the individuals’ mouth and biofilm
formation on resins was measured and compared. Finally, the data were analyzed using Kruskal-Wallis and Chi-square tests.
Results:The observed differences between unmodified acrylic resin (control) and acrylic resin with nano-chitosan
particles in terms of biofilm formation were significant (P<0.05), but no significant difference was found in the formation
of biofilm species on resins.
Conclusion: Biofilm formation of Candida species depends on acrylic resin type, in a way that by adding nano-chitosan
particles to acrylic resins, biofilm formation of Candida species was significantly reduced. To decrease the organization
of biofilm and denture stomatitis, the use of acrylics with nano-chitosan particles in producing dentures is recommended.