Volume 3, Issue 1 (March 2017)                   Curr Med Mycol 2017, 3(1): 6-12 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shirani M, Samimi A, Kalantari H, madani M, Kord Zanganeh A. Chemical composition and antifungal effect of hydroalcoholic extract of Allium tripedale (Tvautv.) against Candida species. Curr Med Mycol. 2017; 3 (1) :6-12
URL: http://cmm.mazums.ac.ir/article-1-138-en.html
Abstract:   (1185 Views)

Background and Purpose: Treatment of life-threatening fungal infections caused by Candida species has become a major problem. Candida spp. are the most important causative agents of candidiasis. Allium tripedale is a medicinal plant that has been traditionally used to treat infections. In the present study, we aimed to determine the chemical compounds and antimicrobial activity of hydroalcoholic extract of A.tripedale against different species of Candida.

Materials and Methods: Phytochemical analysis was performed to identify the possible bioactive components of this extract by using gas chromatography and mass spectroscopy (GC-MS). The hydroalcoholic extract of A. tripedale were collected.Different concentrations of A. tripedale (50, 25, 12.5, and 6.25 mg/ml) were used to evaluate its antifungal activity against Candida species (C. albicans, C. parapsilosis,and C. krusei) using disk diffusion assay.
Results: The GC-MS analysis revealed the presence of 40 different phytoconstituents with peak area; the major compounds were tetracosane, hexadecanoic acid, 1-eicosanol, 1,2-dihydro-pyrido[3,2,1-kl]phenothiazin-3-one, 2-hexadecen-1-ol, and 3,7,11,15-tetramethyl. Hydroalcoholic extract showed strong antimicrobial activity (inhibition zone ⩾ 20 mm), moderate antimicrobial activity (inhibition zone < 12-20 mm), and no inhibition (zone < 12 mm). In addition, the hydroalcoholic extract exhibited the highest antimicrobial properties against C. albicans strains.
Conclusion: A. tripedale extract had a considerable inhibitory effect against various Candida species, but its highest inhibitory effect was against Candid albicans. Further investigations are required to detect the performance of this plant in the treatment of Candida infection.
 

Full-Text [PDF 738 kb]   (457 Downloads)    
Type of Study: Original Articles | Subject: Medical Mycology
Received: 2017/06/7 | Accepted: 2017/08/5 | Published: 2017/12/12

References
1. Ramesh P, Okigbo RN. Effects of plants and medicinal plant combinations as anti-infectives. Afr J Pharm Pharmacol. 2008; 2(7):130-5.
2. Arif T, Bhosale JD, Kumar N, Mandal TK, Bendre RS, Lavekar GS, et al. Natural products–antifungal agents derived from plants. J Asian Nat Prod Res. 2009;11(7):621-38. [DOI:10.1080/10286020902942350]
3. Hashemi SJ, Asgarpanah J, Alaee Z, Sadeghian S, Hasani H, Azimi A. In vitro antifungal activity of four medicinal plants used in Iranian Traditional Medicine. Res J Pharm.2014; 1(1):39-43.
4. Rios JL, Recio MC. Medicinal plants and antimicrobial activity. J Ethnopharmacol. 2005; 100(1-2):80-4. [DOI:10.1016/j.jep.2005.04.025]
5. Kazemi S, Asgary S, Moshtaghian J, Rafieian M, Adelnia A, Shamsi F. Liver-protective effects of hydroalcoholic extract of allium hirtifolium boiss. In rats with alloxaninduced diabetes mellitus. ARYA Atheroscler. 2010;6(1):11-5.
6. Mnayer D, Fabiano-Tixier AS, Petitcolas E, Hamieh T,Nehme N, Ferrant C, et al. Chemical composition,antibacterial and antioxidant activities of six essentials oils from the Alliaceae family. Molecules. 2014;19(12):20034-53. [DOI:10.3390/molecules191220034]
7. Lazarević JS, Ðorđević AS, Zlatković BK, Radulović NS, Palić RM. Chemical composition and antioxidant and antimicrobial activities of essential oil of Allium sphaerocephalon L. subsp. sphaerocephalon (Liliaceae) inflorescences. J Sci Food Agric. 2011; 91(2):322-9. [DOI:10.1002/jsfa.4189]
8. Pfaller MA, Diekema DJ. Epidemiology of invasive Candidiasis: a persistent public health problem. Clin Microbiol Rev. 2007; 20(1):133-63. [DOI:10.1128/CMR.00029-06]
9. Rehman A, Rehman A, Ahmad I. Antibacterial,antifungal, and insecticidal potentials of Oxalis corniculata and its isolated compounds. Int J Anal Chem.2015; 2015:842468. [DOI:10.1155/2015/842468]
10. Corsello S, Spinillo A, Osnengo G, Penna C, Guaschino S, Beltrame A, et al. An epidemiological survey of vulvovaginal candidiasis in Italy. Eur J Obstet Gynaecol Reprod Biol. 2003; 110(1):66-72. [DOI:10.1016/S0301-2115(03)00096-4]
11. Richter SS, Galask RP, Messer SA, Hollis RJ, Diekema DJ, Pfaller MA. Antifungal susceptibilities of Candida species causing vulvovaginitis and epidemiology of recurrent cases. J Clin Microbiol. 2005; 43(5):2155-62. [DOI:10.1128/JCM.43.5.2155-2162.2005]
12. Nasrollahi Z, Yadegari MH, Roudbar Mohammadi S,Roudbary M, Hosseini Poor M, Nikoomanesh F, et al.Fluconazole resistance Candida albicans in females with recurrent Vaginitis and Pir1 overexpression. Jundishapur J Microbiol. 2015; 8(9):e21468. [DOI:10.5812/jjm.21468]
13. Dabur R, Singh H, Chhillar AK, Ali M, Sharma GL.Antifungal potential of Indian medicinal plants. Fitoterapia. 2004; 75(3-4):389-91. [DOI:10.1016/j.fitote.2004.01.015]
14. Das K, Tiwari RK, Shrivastava DK. Techniques for evaluation of medicinal plant products as antimicrobial agents: current methods and future trends. J Med Plants Res. 2010; 4(2):104-11 .
15. Forbes BA, Sahm DF, Weissfeld AS. Diagnostic microbiology. Bailey Scott Diagn Microbiol. 2002;11(1):11-4.
16. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitr evaluating antimicrobial activity: a review. J Pharm Biomed Anal. 2016; 6(2):71-9. [DOI:10.1016/j.jpha.2015.11.005]
17. Kim HJ, Suh HJ, Lee CH, Kim JH, Kang SC, Park S, et al. Antifungal activity of glyceollins isolated from soybean elicited with Aspergillus sojae. J Agric Food Chem. 2010; 58(17):9483-7. [DOI:10.1021/jf101694t]
18. Sojakova M, Liptajova D, Borovsky M, Subik J. Fluconazole and itraconazole susceptibility of vaginal yeast isolates from Slovakia. Mycopathologia. 2004;157(2):163-9. [DOI:10.1023/B:MYCO.0000020594.35357.b0]
19. Kirkpatrick WR, Turner TM, Fothergill AW, McCarthy DI, Redding SW, Rinaldi MG, et al. Fluconazole disk diffusion susceptibility testing of Candida species. J Clin Microbiol. 1998; 36(11):3429-32.
20. Brown GD, Denning DW, Gow NA, Levitz SM, Netea MG, White TC. Hidden killers: human fungal infections.Sci Transl Med. 2012; 4(165):165rv13. [DOI:10.1126/scitranslmed.3004404]
21. Amjad L, Rezvani Z, Madani M. The effect of methanolic extract of anthemis gayana on Candida Spp. Intl J Agric Crop Sci. 2013; 5(10):1140.
22. Abad MJ, Ansuategui M, Bermejo P. Active antifungal substances from natural sources. Arkivoc. 2007; 7(11):116-45.
23. Shams-Ghahfarokhi M, Shokoohamiri MR, Amirrajab N, Moghadasi B, Ghajari A, Zeini F, et al. In vitro antifungal activities of Allium cepa, Allium sativum and ketoconazole against some pathogenic yeasts and dermatophytes. Fitoterapia. 2006; 77(4):321-3. [DOI:10.1016/j.fitote.2006.03.014]
24. Amin M, Kapadnis BP. Heat stable antimicrobial activity of Allium ascalonicum against bacteria and fungi. Indian J Exp Biol. 2005; 43(8):751-4 .
25. Dandekar R, Fegade B, Bhaskar VH. GC-MS analysis of phytoconstituents in alcohol extract of Epiphyllum oxypetalum leaves. J Pharm Phytochem. 2015; 4(1):149-54.
26. Theng KB, Korpenwar AN. Phytochemical analysis of ethanol extract of ampelocissus latifolia (Roxb.) planch tuberous root using UV-vis, ftir and GC-MS. Int J Pharm Sci Res. 2015; 6(9):3936.
27. Anjusha S, Gangaprasad A, Radhamany PM. GC-MS analysis of leaf extract of gynochthodes umbellata (L.) razafim. and b. bremer (Rubiaceae). Int J Pharm Sci Res. 2015; 6(11):4826.
28. Zelenitsky SA, Zhanel GG. Phenazopyridine in urinary tract infections. Ann Pharmacother. 1996; 30(7-8):866-8. [DOI:10.1177/106002809603000727]
29. Choojun S, Yoonprayong P. Improvement of propionic acid production for antifungal activity from whey by calcium alginate immobilization of propionibacterium acidipropionici TISTR 442. J Agric Sci Tech. 2012; 2(7A):863.
30. Chandrasekar T, Rao MR, Kumar RV, Prabhu K, Nandha Kumar S, Divya D. GC–MS analysis, antimicrobial, antioxidant activity of an Ayurvedic medicine, Nimbapatradi Choornam. J Chem Pharm Res. 2015; 7(8):124-36.

Add your comments about this article : Your username or Email:
CAPTCHA code

Send email to the article author


© 2015 All Rights Reserved | Current Medical Mycology