Volume 3, Issue 1 (March 2017)                   Curr Med Mycol 2017, 3(1): 1-5 | Back to browse issues page

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Ghiaie Asl I, Motamedi M, Shokuhi G R, Jalalizand N, Farhang A, Mirhendi H. Molecular characterization of environmental Cladosporium species isolated from Iran. Curr Med Mycol. 2017; 3 (1) :1-5
URL: http://cmm.mazums.ac.ir/article-1-130-en.html
Abstract:   (1286 Views)
Background and Purpose: Cladosporium species are ubiquitous, saprobic,dematiaceous fungi, only infrequently associated with human and animal opportunisticinfections.
Materials and Methods: Airborne samples were collected using the settle plate method, and soil samples were obtained from a depth of 5-10 cm of the superficial soil layer. Samples were cultured on Sabouraud dextrose agar (SDA) plates, incubated at 25°C, and examined daily for fungal colonies for two to three weeks. Isolates were identified as Cladosporium species according to the macroscopic and microscopic criteria. For species differentiation, DNA from 53 isolates was extracted and subjected to amplification of the internal transcribed spacer (ITS) region followed by sequencing.
Results: A total of 270 samples were collected from various environmental sources, of which 79 strains of Cladosporium species were isolated. The most frequent species was C. cladosporioides (50.6%), followed by C. iridis (44.3%), C. elatum (2.5%), C. peranqestum (1.3%), and C. alicinum. (1.3%).
Conclusion: The collected data can serve as baseline information for future research and may be useful in the development of preventive and educational strategies.

Full-Text [PDF 552 kb]   (631 Downloads)    
Type of Study: Original Articles | Subject: Environmental Mycology
Received: 2017/04/26 | Accepted: 2017/07/9 | Published: 2017/07/12

1. Ellis MB. Dematiaceous hyphomycetes. London:Commonwealth Mycological Institute; 1971.
2. Flannigan B, Samson RA, Miller JD. Microorganisms in home and indoor work environments: diversity, health impacts, investigation and control. Florida, USA: CRC Press; 2011. [DOI:10.1201/b10838]
3. Schubert K. Morphotaxonomic revision of foliicolous Cladosporium species (hyphomycetes). Halle (Saale), Germany: Martin Luther-Universität Halle-Wittenberg;2005.
4. Simon-Nobbe B, Denk U, Pöll V, Rid R, Breitenbach M.The spectrum of fungal allergy. Int Arch Allergy Immunol. 2008; 145(1):58-86. [DOI:10.1159/000107578]
5. Queiroz-Telles F, Esterre P, Perez-Blanco M, Vitale RG, Salgado CG, Bonifaz A. Chromoblastomycosis: an overview of clinical manifestations, diagnosis and treatment. Med Mycol. 2009; 47(1):3-15. [DOI:10.1080/13693780802538001]
6. Siqueira VM, Oliveira H, Santos C, Paterson RR, Gusmao NB, Lima N. Filamentous fungi in drinking water, particularly in relation to biofilm formation. Int J Environ Res Public Health. 2011; 8(2):456-69. [DOI:10.3390/ijerph8020456]
7. Achatz G, Oberkofler H, Lechenauer E, Simon B, Unger A, Kandler D, et al. Molecular cloning of major and minor allergens of Alternaria alternata and Cladosporium herbarum. Mol Immunol. 1995; 32(3):213-27. [DOI:10.1016/0161-5890(94)00108-D]
8. Bensch K, Braun U, Groenewald JZ, Crous PW. The genus cladosporium. Stud Mycol. 2012; 72(1):1-401. [DOI:10.3114/sim0003]
9. Amirmijani A, Khodaparast SA, Zare R. Additions to the knowledge of the genus Cladosporium in Iran. Mycol Iran. 2015; 2(1):11-21.
10. Azimi F, Naddafi K, Nabizadeh R, Hassanvand MS, Alimohammadi M, Afhami S, et al. Fungal air quality in hospital rooms: a case study in Tehran, Iran. J Environ Health Sci Eng. 2013; 11(1):30. [DOI:10.1186/2052-336X-11-30]
11. Aghamirian MR, Ghiasian SA. The prevalence of fungi in soil of Qazvin, Iran. Jundishapur J Microbiol. 2012; 6(1):76-9. [DOI:10.5812/jjm.4591]
12. Ho MH, Castaneda RF, Dugan FM, Jong SC.Cladosporium and Cladophialophora in culture: descriptions and an expanded key. Mycotaxon.1999; 72:115-57.
13. Sandoval-Denis M, Sutton DA, Martin-Vicente A, Cano-Ghiaie Asl I et al. Molecular Characterization of Cladosporium Isolates Curr Med Mycol, 2017, 3(1): 1-5 5 Lira JF, Wiederhold N, Guarro J, et al. Cladosporium species recovered from clinical samples in the United States. J Clin Microbiol. 2015; 53(9):2990-3000. [DOI:10.1128/JCM.01482-15]
14. Haddadi S, Khosravi AR, Mina R, Masoudi N, Saeed S,Minoo S. Molecular typing of Iranian cladosporium isolates using RAPD-PCR. Biotechnology. 2008; 7(4):763-8. [DOI:10.3923/biotech.2008.763.768]
15. Rezaei-Matehkolaei A, Makimura K, de Hoog S,Shidfar MR, Zaini F, Eshraghian M, et al.Molecular epidemiology of dermatophytosis in Tehran, Iran, a clinical and microbial survey. Med Mycol. 2013; 51(2):203-7. [DOI:10.3109/13693786.2012.686124]
16. Yamada Y, Makimura K, Merhendi H, Ueda K,Nishiyama Y, Yamaguchi H, et al. Comparison of different methods for extraction of mitochondrial DNA from human pathogenic yeasts. Jpn J Infect Dis. 2002; 55(4):122-5.
17. van den Ende AG, De Hoog G. Variability and molecular diagnostics of the neurotropic species Cladophialophora bantiana. Stud Mycol. 1999; 43:151-62.
18. Revankar SG, Sutton DA. Melanized fungi in human disease. Clin Microbiol Rev. 2010; 23(4):884-928. [DOI:10.1128/CMR.00019-10]
19. Jafari A FM, Rajibon H. Study on air fungal flora contamination of operation rooms in Yazd Hospitals. 5th National Iranian Congress of Parasitology 15-17 November 2005; Shahid Beheshti University, Tehran, Iran; 2005.
20. Hedayati MT, Mohammadpour RA. A survey on the mycological contamination of the air and the equipment of operating rooms of 17 hospitals. J Med Facul Gilan Univ Med Sci. 1999; 8(19):56-61.
21. Nourian A, Badali H. A survey of spores of fungal agents in operating-room equipments and special care units in Zanjan hospitals 2001. J Zanjan Univ Med Sci Health Serv. 2001; 9(36):9-16 (Persian).
22. Sepahvand A, Shams-Ghahfarokhi M, Allameh A, Razzaghi-Abyaneh M. Diversity and distribution patterns of airborne microfungi in indoor and outdoor hospital environments in Khorramabad, Southwest Iran. Jundishapur J Microbiol. 2013; 6(2):186-92. [DOI:10.5812/jjm.5074]
23. Hoseinzadeh E, Taghavi M, Samarghandie MR. Evaluation of fungal and bacterial aerosols in the different wards of Malayer city's hospitals in 2011-2012. J Hospital. 2014; 13(3):99-108.
24. Nourian AA, Badali H, Khodaverdi M, Hamzehei H, Mohseni S. Airborne mycoflora of Zanjan-Iran. Int J Agric Biol. 2007; 9(4):628-30.
25. Boudier HS. Guest Commentary. The pharmacology of angiogenesis in cardiovascular disease. Dial Cardiovascul Med. 2001; 6(3):143-220.
26. Curtis MD, Gore J, Oliver RP. The phylogeny of the tomato leaf mould fungus Cladosporium fulvum syn. Fulvia fulva by analysis of rDNA sequences. Curr Genet.1994; 25(4):318-22. [DOI:10.1007/BF00351484]
27. Zeng QY, Westermark SO, Rasmuson-Lestander A, Wang XR. Detection and quantification of Cladosporium in aerosols by real-time PCR. J Environ Monit. 2006; 8(1):153-60. [DOI:10.1039/B509515H]
28. Vries GD. Contribution to the knowledge of the genus Cladosporium Link ex Fr. [PhD Thesis]. Utrecht, Netherlands: University of Utrecht; 1952.
29. Kwon-Chung K, Schwartz IS, Rybak BJ. A pulmonary fungus ball produced by Cladosporium cladosporioides. Am J Clin Pathol. 1975; 64(4): 564-8. [DOI:10.1093/ajcp/64.4.564]
30. Polack F, Siverio C, Bresky RH. Corneal chromomycosis: double infection by Phialophora verrucosa (Medlar) and Cladosporium cladosporioides (Frescenius). Ann Ophthalmol. 1976; 8(2):139-44.
31. Gugnani H, Gupta S, Talwar RS. Role of opportunistic fungi in ocular infections in Nigeria. Mycpah. 1978; 65(1-3):155-66. [DOI:10.1007/BF00447186]
32. Vieira MR, Milheiro A, Pacheco FA. Phaeohyphomycosis due to Cladosporium cladosporioides. Med Mycology. 2001; 39(1):135-7. https://doi.org/10.1080/714030986 [DOI:10.1080/mmy.]

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