Volume 3, Issue 2 (June 2017)                   Curr Med Mycol 2017, 3(2): 20-25 | Back to browse issues page


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Abstract:   (594 Views)
Background and Purpose: Cryptococcus species are pathogenic and non-pathogenic basidiomycete yeasts that are found widely in the environment. Based on phenotypic methods, this genus has many species; however, its taxonomy is presently being re- evaluated by modern techniques. The Cryptococcus species complex includes two sibling taxa of Cryptococcus neoformans and Cryptococcus gattii. We aimed to investigate the possible distribution of Cryptococcus species in pigeon nests and Eucalyptus trees in Ilam, Iran, using molecular techniques.
Materials and Methods: Two hundred and seventy-four specimens were collected from pigeon nests and Eucalyptus trees during 2016-2017. All the specimens were sub- cultured on Sabouraud Glucose Agar with chloramphenicol and bird seed agar. For molecular identification, the ITS1-5.8S-ITS2 rDNA region was amplified using the first and fourth internal transcribed spacer (ITS1 and ITS4, respectively) primers. The purified products were applied for cycle sequencing reactions in forward direction with ITS1 primer. The obtained results were analyzed with Chromas 2.3.
Results: Thirty-three out of 186 cultures (17.7%) and 11 out of 88 cultures (12.5%) were positive among pigeon nest and Eucalyptus tree specimens, respectively. Cryptococcus albidus (17.2%), C. albidus var. kuetzingii (3.4%), C. adeliensis (3.4%), C. uzbekistanensis (3.4%), and C. neoformans var. grubii (3.4%) were isolated from pigeon nests, and Cryptococcus adeliensis (25%) was the only Cryptococcus species isolated from Eucalyptus trees.
Conclusion: The presence of pigeons and Eucalyptus trees in the vicinity of some particular places such as rest homes and hospitals should be considered as a risk factor for the immunocompromised population.  
Full-Text [PDF 432 kb]   (275 Downloads)    
Type of Study: Original Articles | Subject: Epidemiology
Received: 2017/09/24 | Accepted: 2017/11/13 | Published: 2018/01/6

References
1. Sanfelice F. Contributo alla morfologia e biologia dei blastomiceti che si sviluppano nei succhi di alcuni frutti. Ann Igien 1894;4:463-95.
2. Takahara DT, Lazéra MdS, Wanke B, Trilles L, Dutra V, Paula DAJd, et al. First report on Cryptococcus neoformans in pigeon excreta from public and residential locations in the metropolitan area of Cuiabá, state of Mato Grosso, Brazil. Rev Inst Med Trop São Paulo 2013;55(6):371-6. [DOI:10.1590/S0036-46652013000600001]
3. Kwon-Chung KJ, Boekhout T, Fell JW, Diaz M. (1557) Proposal to conserve the name Cryptococcus gattii against C. hondurianus and C. bacillisporus (Basidiomycota, Hymenomycetes, Tremellomycetidae). Taxon 2002;51(4):804-6.
4. Hagen F, Lumbsch HT, Arsenijevic VA, Badali H, Bertout S, Billmyre RB, et al. Importance of resolving fungal nomenclature: the case of multiple pathogenic species in the Cryptococcus genus. Msphere 2017;2(4):e00238-17. [DOI:10.1128/mSphere.00238-17]
5. Emmons CW. Saprophytic Sources of Cryptococcus neoformans associated with the Pigeon (Columba livia). Am J Hyg 1955;62(3):227-32. [DOI:10.1093/oxfordjournals.aje.a119775]
6. Kwon-Chung KJ, Fraser JA, Doering TL, Wang ZA, Janbon G, Idnurm A, et al. Cryptococcus neoformans and Cryptococcus gattii, the etiologic agents of cryptococcosis. Cold Spr Har Pers Med 2014;4(7): a019760. [DOI:10.1101/cshperspect.a019760]
7. Ajello L. Occurrence of Cryptococcus neoformans in soils. Am J Hyg 1958;67(1):72-7.
8. Springer DJ, Billmyre RB, Filler EE, Voelz K, Pursall R, Mieczkowski PA, et al. Cryptococcus gattii VGIII isolates causing infections in HIV/AIDS patients in Southern California: identification of the local environmental source as arboreal. PLoS Path 2014;10(8):e1004285. [DOI:10.1371/journal.ppat.1004285]
9. Ma H, Hagen F, Stekel DJ, Johnston SA, Sionov E, Falk R, et al. The fatal fungal outbreak on Vancouver Island is characterized by enhanced intracellular parasitism driven by mitochondrial regulation. Proc Nat Acad Sci 2009;106(31):12980-5. [DOI:10.1073/pnas.0902963106]
10. Soltani M, Bayat M, Hashemi SJ, Zia M, Pestechian N. Isolation of Cryptococcus neoformans and other opportunistic fungi from pigeon droppings. Journal of Research in Medical Sciences: J Res Med Sci 2013;18(1):56.
11. Hedayati MT, Mayahi S, Fakhar M, Shokohi T, Majidi M. Cryptococcus neoformans isolation from swallow (Hirundo rustica) excreta in Iran. Rev Ins Med Trop São Paulo 2011;53(3):125-7. [DOI:10.1590/S0036-46652011000300002]
12. Khosravi A. Isolation of Cryptococcus neoformans from pigeon (Columba livia) droppings in northern Iran. Mycopathologia 1997;139(2):93-5. [DOI:10.1023/A:1006863705759]
13. Badali H, Alian S, Fakhim H, Falahatinejad M, Moradi A, Mohammad Davoudi M, et al. Cryptococcal meningitis due to Cryptococcus neoformans genotype AFLP1/VNI in Iran: a review of the literature. Mycoses 2015;58(12):689-93. [DOI:10.1111/myc.12415]
14. Salehei Z, Mahmoudabadi AZ, Zarrin M. Lack of Cryptococcus gattii from Eucalyptus in Ahvaz. Cur Med Mycol 2015;1(1):1-3. [DOI:10.18869/acadpub.cmm.1.1.1]
15. Shields AB, Ajello L. Medium for selective isolation of Cryptococcus neoformans. Sci 1966;151(3707):208-9. [DOI:10.1126/science.151.3707.208]
16. Kidd SE, Chow Y, Mak S, Bach PJ, Chen H, Hingston AO, et al. Characterization of environmental sources of the human and animal pathogen Cryptococcus gattii in British Columbia, Canada, and the Pacific Northwest of the United States. App Environ Microbiol 2007;73(5):1433-43. [DOI:10.1128/AEM.01330-06]
17. DeBess E, Lockhart SR, Iqbal N, Cieslak PR. Isolation of Cryptococcus gattii from Oregon soil and tree bark, 2010–2011. BMC Microbiol 2014;14(1):323. [DOI:10.1186/s12866-014-0323-2]
18. Silva GAd, Bernardi TL, Schaker PDC, Menegotto M, Valente P. Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification. Brazilian Arch Biol Technol 2012;55(2):319-27. [DOI:10.1590/S1516-89132012000200020]
19. Mohammadi A, Hashemi SM, Abtahi SH, Lajevardi SM, Kianipour S, Mohammadi R. An investigation on non-invasive fungal sinusitis; Molecular identification of etiologic agents. J Res Med Sci 2017;22: 67. [DOI:10.4103/jrms.JRMS_166_17]
20. Zarrinfar H, Kaboli S, Dolatabadi S, Mohammadi R. Rapid detection of Candida species in bronchoalveolar lavage fluid from patients with pulmonary symptoms. Brazilian J Microbiol 2016;47(1):172-6. [DOI:10.1016/j.bjm.2015.02.001]
21. Mitchell TG, Casta-eda E, Nielsen K, Wanke B, Lazéra MS. Environmental niches for Cryptococcus neoformans and Cryptococcus gattii. Cryptococcus: Am Soci Microbiol 2011. p. 237-59. [DOI:10.1128/9781555816858.ch18]
22. Scorzetti G, Petrescu I, Yarrow D, Fell JW. Cryptococcus adeliensis sp. nov., a xylanase producing basidiomycetous yeast from Antarctica. Antonie van Leeuwenhoek 2000;77(2):153-7. [DOI:10.1023/A:1002124504936]
23. Rimek D, Haase G, Lück A, Casper J, Podbielski A. First report of a case of meningitis caused by Cryptococcus adeliensis in a patient with acute myeloid leukemia. J Clin Microbiol 2004;42(1):481-3. [DOI:10.1128/JCM.42.1.481-483.2004]
24. Tintelnot K, Losert H. Isolation of Cryptococcus adeliensis from clinical samples and the environment in Germany. J Clin Microbiol 2005;43(2):1007. [DOI:10.1128/JCM.43.2.1007.2005]
25. Borhani B, Rahimian H. Cryptococcus adeliensis inciting branch canker on stone fruit trees. Eur J Plant Pathol 2016;145(1):71-80. [DOI:10.1007/s10658-015-0814-5]
26. Borhani B, Rahimian H, Babaeizad V, Zohour E. Cryptococcus adeliensis a yeast species inciting stem canker on stone fruit trees. J Plant Pathol 2013;95(3):659-668.
27. Huang Y-H, Lin I-H, Chang T-C, Tseng S-H. Early Diagnosis and Successful Treatment of Cryptococcus albidus Keratitis: A Case Report and Literature Review. Med 2015;94(19): e885. [DOI:10.1097/MD.0000000000000885]
28. Liu Y, Ma S, Wang X, Xu W, Tang J. Cryptococcus albidus encephalitis in newly diagnosed HIV-patient and literature review. Med Mycol case rep 2014;3:8-10. [DOI:10.1016/j.mmcr.2013.11.002]
29. Ragupathi L, Reyna M. Case report of Cryptococcus albidus peritonitis in a peritoneal dialysis patient and a review of the literature. Per Dial Inter 2015;35(4):421-7. [DOI:10.3747/pdi.2013.00134]
30. Fonseca Á, Scorzetti G, Fell JW. Diversity in the yeast Cryptococcus albidus and related species as revealed by ribosomal DNA sequence analysis. Canadian J Microbiol 1999;46(1):7-27. [DOI:10.1139/w99-108]
31. Powel MS, Alizadeh AA, Budvytiene I, Schaenman JM, Banaei N. First isolation of Cryptococcus uzbekistanensis from an immunocompromised patient with lymphoma. J Clin Microbiol 2012;50(3):1125-7. [DOI:10.1128/JCM.05678-11]
32. Lyles MB, Fredrickson HL, Bednar AJ, Fannin HB, Sobecki TM, Griffin DW. Medical geology: Dust exposure and potential health risks in the Middle East: International Center for Remote Sensing of Environment; 2011.
33. Kwon-Chung K. Perfect state of Cryptococcus uniguttulatus. Inter J Syst Evol Microbiol 1977;27(3):293-9. [DOI:10.1099/00207713-27-3-293]
34. Kreger-van Rij N. Taxonomy of Cryptococcus Neoformans and its variety Uniguttulatus. Antonie van Leeuwenhoek 1961;27(1):59-64. [DOI:10.1007/BF02538423]
35. Kwon-Chung KJ, Boekhout T, Wickes BL, Fell JW. Systematics of the genus Cryptococcus and its type species C. neoformans. Cryptococcus: Am Soci Microbiol 2011. p. 3-15.
36. Day JN, Qihui S, Thanh LT, Trieu PH, Van AD, Thu NH, et al. Comparative genomics of Cryptococcus neoformans var. grubii associated with meningitis in HIV infected and uninfected patients in Vietnam. PLoS Negl Trop Dis 2017;11(6):e0005628. [DOI:10.1371/journal.pntd.0005628]
37. Afshari SAK, Shokohi T, Aghili R, Badali H. Epidemiology and molecular characterization of Cryptococcus neoformans isolated from pigeon excreta in Mazandaran province, northern Iran. J de Mycol Méd 2012;22(2):160-6. [DOI:10.1016/j.mycmed.2012.02.002]
38. Dou H, Wang H, Xie S, Chen X, Xu Z, Xu Y. Molecular characterization of Cryptococcus neoformans isolated from the environment in Beijing, China. Med Mycol 2017:myx026. [DOI:10.1093/mmy/myx026]