Volume 3, Issue 4 ( December 2017)                   Curr Med Mycol 2017, 3(4): 1-5 | Back to browse issues page

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Noori M, Dakhili M, Sepahvand A, Davari N. Evaluation of esterase and hemolysin activities of different Candida species isolated from vulvovaginitis cases in Lorestan Province, Iran. Curr Med Mycol. 2017; 3 (4) :1-5
URL: http://cmm.mazums.ac.ir/article-1-161-en.html
Abstract:   (1084 Views)
Background and Purpose: Annually affecting millions of women, vulvovaginal candidiasis (VVC) is commonly described by signs and symptoms of vulvovaginal inflammation in the presence of Candida species. Today, the detection of the virulence factors plays a major role in the understanding of pathogenesis of candidiasis and helps produce new anticandidial drugs to improve its treatment efficiency. Herein, we aimed to evaluate the esterase and hemolysin activities of the vaginal isolates of Candida and their relationship with the presence of VVC.
Materials and Methods: One-hundred vaginal clinical specimens were randomly collected during September-December 2016. The target population consisted of married women suspected of VVC who presented to health centers in Lorestan Province, Iran. In this study, the esterase activity and hemolysin production of Candida clinical isolates were evaluated using the Tween 80 opacity test and the plate assay, respectively.
Results: The most frequent Candida species was C. albicans (66; 66%), followed by C. glabrata (11; 11%) and C. tropicalis (11; 11%). The highest esterase activity was found in C. krusei (75%), followed by C. albicans (68.2%) and C. glabrata (54.5%). The greater part of the positive esterase isolates had Pz 4+ scores. Among the Candida species, C. albicans (22.7%), C. glabrata (63.6%), and C. krusei (50%) were found to have the highest rates of alpha, beta, and gamma hemolysin production, respectively. The level of hemolytic activity in 51% of the Candida species was Pz 4+ scores.
Conclusion: According to our results, the higher expression rates of both enzymes in C. albicans species relative to those of non-albicans Candidate species can partly reflect the role of the virulence factors involved in C. albicans pathogenicity.
Full-Text [PDF 322 kb]   (487 Downloads)    
Type of Study: Original Articles | Subject: Medical Mycology
Received: 2017/09/30 | Accepted: 2018/02/17 | Published: 2018/03/11

1. Spence D. Candidiasis (vulvovaginal). BMJ Clin Evid. 2015; 2015:815.
2. Pfaller MA, Diekema DJ. Epidemiology of invasive candidiasis: a persistent public health problem. Clin Microbiol Rev. 2007; 20(1):133-63. [DOI:10.1128/CMR.00029-06]
3. Falahati M, Sepahvand A, Mahmoudvand H, Baharvand P, Jabbarnia S, Ghojoghi A, et al. Evaluation of the antifungal activities of various extracts from Pistacia atlantica Desf. Curr Med Mycol. 2015; 1(3):25-32. [DOI:10.18869/acadpub.cmm.1.3.25]
4. Sobel JD. Vulvovaginal Candidiasis. Lancet. 2007;369(9577):1961-71. [DOI:10.1016/S0140-6736(07)60917-9]
5. Calderone RA, Fonzi WA. Virulence factors of Candida albicans. Trends Microbiol. 2001; 9(7):327-35. [DOI:10.1016/S0966-842X(01)02094-7]
6. Cutler JE. Putative virulence factors of Candida albicans. Ann Rev Microbiol. 1991; 45:187-218. [DOI:10.1146/annurev.mi.45.100191.001155]
7. Deorukhkar SC, Saini S, Mathew S. Virulence factors contributing to pathogenicity of Candida tropicalis and its antifungal susceptibility profile. Int J Microbiol.2014; 2014:456878. [DOI:10.1155/2014/456878]
8. Ghannoum MA. Potential role of phospholipases in virulence and fungal pathogenesis. Clin Microbiol Rev. 2000; 13(1):122-43. [DOI:10.1128/CMR.13.1.122-143.2000]
9. Rudek WA. Esterase activity in Candida species. J Clin Microbiol. 1978; 8(6):756-9.
10. Watanabe T, Takano M, Murakami M, Tanaka H,Matsuhisa A, Nakao N, et al. Characterization of a haemolytic factor from Candida albicans. Microbiology. 1999; 145(Pt 3):689-94. [DOI:10.1099/13500872-145-3-689]
11. Pakshir K, Zomorodian K, Karamitalab M, Jafari M,Taraz H, Ebrahimi H. Phospholipase, esterase and hemolytic activities of Candida spp. isolated from onychomycosis and oral lichenplanus lesions. J Mycol Med. 2013; 23(2):113-8. [DOI:10.1016/j.mycmed.2013.04.007]
12. Mohammadi R, Mirhendi H, Rezaei-Matehkolaei A, et al. Molecular identification and distribution profile of Candida species isolated from Iranian patients. Med Mycol 2013;51:657—63. [DOI:10.3109/13693786.2013.770603]
13. Shirkhani S, Sepahvand A, Mirzaee M, Anbari K. Phospholipase and proteinase activities of Candida spp. isolates from vulvovaginitis in Iran. J Mycol Med. 2016;26(3):255-60. [DOI:10.1016/j.mycmed.2016.05.001]
14. Fatahinia M, Poormohamadi F, Mahmoudabadi AZ. Comparative study of esterase and hemolytic activities in clinically important Candida species, isolated from oral cavity of diabetic and non-diabetic individuals. Jundishapur J Microbiol 2015;8.(3):e20893. [DOI:10.5812/jjm.20893]
15. Tsang CS, Chu FC, Leung WK, Jin LJ, Samaranayake LP, Siu SC. Phospholipase proteinase and haemolytic activities of Candida albicans isolated from oral cavities of patients with type 2 diabetes mellitus. J Med Microbiol. 2007; 56(Pt 10):1393-8. [DOI:10.1099/jmm.0.47303-0]
16. Manns JM, Mosser DM, Buckley HR. Production of a hemolytic factor by Candida albicans. Infect Immun 1994;62:5154—6.
17. Asadi MA, Rasti S, Arbabi M, Hooshyar H, Yoosefdoost H, 1997, Prevalence of vaginal Candidiasis in married women referred to Kashan's health centers, 1993-94, Feyz, 1(1):21-27.
18. Akbarzadeh M, Bonyadpoure B, Pacshir K, Mohagheghzadeh A, 2010, Causes and clinical symptoms of vaginal candidiasis in patients referring to selective clinics of Shiraz University of Medical Sciences (2009) Arak University of Medical Sciences Journal, 13 (3):12-20.
19. Faraji, R, Rahimi1, M A, Rezvanmadani, F, Hashemi, M, 2012, Prevalence of vaginal candidiasis infection in diabetic women, African Journal of Microbiology Research, 6(11): 2773-8.
20. Fatahinia M, Halvaeezadeh M, Rezaei-Matehkolaei A. Comparison of enzymatic activities in different Candida species isolated from womenwith vulvovaginitis. J Mycol Med. 2017;27(2):188-94. [DOI:10.1016/j.mycmed.2017.01.009]
21. Martens MG, Hoffman P, El-Zaatari M. Fungal species changes in the female genital tract. J Low Genit Tract Dis. 2004;8(1):21-4. [DOI:10.1097/00128360-200401000-00006]
22. Ramesh, N, Priyadharsini, M, Sumathi, C S, Balasubramanian, V, Hemapriya, J, Kannan, R, 2011, Virulence Factors and Anti Fungal Sensitivity Pattern of Candida Sp Isolated from HIV and TB Patients, Indian J Microbiol, 51(3):273-8. [DOI:10.1007/s12088-011-0177-3]
23. Yücesoy, M, Marol S, 2003, Determination of esterase activity of Candida species, Mikrobiyoloji Bulteni, 37 (1): 59-63.
24. Kumar C P, Menon T, Sundararajan T, Nalini S, Thirunarayan M A, Rajasekaran S, 2006, Esterase activity of Candida species isolated from immunocompromised hosts, Revista Iberoamericana de Micologia, 23(2):101-103.
25. Malcok H K, Aktas E, Ayyildiz A, Yigit N, Yazgi H, 2009, Hemolytic Activities of the Candida Species in Liquid Medium Eurasian J Med August; 41(2): 95–98 .

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