Volume 3, Issue 4 ( December 2017)                   Curr Med Mycol 2017, 3(4): 15-20 | Back to browse issues page

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Badali H, Rezaie S, Meis J F, Agha Kuchak Afshari S, Modiri M, Hagen F, et al . Microsatellite Genotyping of Candida parapsilosis Clinical Isolates . Curr Med Mycol. 2017; 3 (4) :15-20
URL: http://cmm.mazums.ac.ir/article-1-178-en.html
Abstract:   (927 Views)
Background and Purpose: Candida parapsilosis is a predominant species found in nosocomial infection, particularly in hospitalized patients. The molecular epidemiology of the clinical strains of this species has not been well studied. The present study was performed with the aim of investigating the microsatellite genotyping of Candida parapsilosis among the Iranian clinical isolates.
Materials and Methods: This study was conducted on 81 independent clinical C. parapsilosis isolates that were genotyped by using a panel of six microsatellite markers.
Results: The short tandem repeat (STR) typing of clinical C. parapsilosis isolates demonstrated 68 separate genotypes, among which 57 genotypes were observed once and the remaining 11 cases were identified for multiple times. The Simpson’s diversity index for the panel of combined six markers yielded a diversity index of 0.9951. The heterogeneity was observed among the Iranian and the Netherlands clinical C. parapsilosis isolates.
Conclusion: As the findings indicated, the clinical C. parapsilosis isolates from Iran showed a high genetic diversity. It can be concluded that molecular epidemiology could be useful for screening during outbreak investigation where C. parapsilosis is involved. 
Full-Text [PDF 1419 kb]   (540 Downloads)    
Type of Study: Original Articles | Subject: Medical Mycology
Received: 2018/01/4 | Accepted: 2018/01/31 | Published: 2018/03/11

1. Pfaller MA, Diekema DJ. Epidemiology of invasive candidiasis: a persistent public health problem. Clin Microbiol Rev. 2007;20(1):133-63. [DOI:10.1128/CMR.00029-06]
2. Laal Kargar M, Fooladi-Rad S, Mohammad Davoodi M, Khalilzadeh S, Hassanzad M, Mayahi S, et al. Fungal colonization in patients with cystic fibrosis. J Mazandaran Univ Med Sci. 2013;22(2):204-18.
3. Bassetti M, Merelli M, Righi E, Diaz-Martin A, Rosello EM, Luzzati R, et al. Epidemiology, species distribution, antifungal susceptibility, and outcome of candidemia across five sites in Italy and Spain. J clin microbiol. 2013;51(12):4167-72. [DOI:10.1128/JCM.01998-13]
4. Nucci M, Queiroz-Telles F, Alvarado-Matute T, Tiraboschi IN, Cortes J, Zurita J, et al. Epidemiology of candidemia in Latin America: a laboratory-based survey. PloS one. 2013;8(3):e59373. [DOI:10.1371/journal.pone.0059373]
5. van Asbeck EC, Clemons KV, Stevens DA. Candida parapsilosis: a review of its epidemiology, pathogenesis, clinical aspects, typing and antimicrobial susceptibility. Crit Rev Microbiol. 2009;35(4):283-309. [DOI:10.3109/10408410903213393]
6. Khodavaisy S, Nabili M, Davari B, Vahedi M. Evaluation of bacterial and fungal contamination in the health care workers' hands and rings in the intensive care unit. J Prev Med Hyg. 2011;52(4):215-8.
7. Pammi M, Holland L, Butler G, Gacser A, Bliss JM. Candida parapsilosis is a significant neonatal pathogen: a systematic review and meta-analysis. Pediatr Infect Dis J. 2013;32(5):e206-16. [DOI:10.1097/INF.0b013e3182863a1c]
8. Levin A, Costa S, Mussi N, Basso M, Sinto S, Machado C, et al. Candida parapsilosis fungemia associated with implantable and semi-implantable central venous catheters and the hands of healthcare workers. Diagn microbiol Infect Dis. 1998;30(4):243-9. [DOI:10.1016/S0732-8893(98)00006-6]
9. Krcmery V, Barnes A. Non-albicans Candida spp. causing fungaemia: pathogenicity and antifungal resistance. J Hosp Infec. 2002;50(4):243-60. [DOI:10.1053/jhin.2001.1151]
10. Trabasso P, Matsuzawa T, Fagnani R, Muraosa Y, Tominaga K, Resende MR, et al. Isolation and drug susceptibility of Candida parapsilosis sensu lato and other species of C. parapsilosis complex from patients with blood stream infections and proposal of a novel LAMP identification method for the species. Mycopathologia. 2015;179(1-2):53-62. [DOI:10.1007/s11046-014-9830-9]
11. Huang Y-C, Lin T-Y, Leu H-S, Peng H-L, Wu J-H, Chang H-Y. Outbreak of Candida parapsilosis fungemia in neonatal intensive care units: Clinical implications and genotyping analysis. Infection. 1999;27(2):97-102. [DOI:10.1007/BF02560505]
12. Desnos-Ollivier M, Bórmida V, Poirier P, Nourrisson C, Pan D, Bretagne S, et al. Population Structure of Candida parapsilosis: No Genetic Difference Between French and Uruguayan Isolates Using Microsatellite Length Polymorphism. Mycopathologia. 2017:1-10.
13. Hays C, Duhamel C, Cattoir V, Bonhomme J. Rapid and accurate identification of species belonging to the Candida parapsilosis complex by real-time PCR and melting curve analysis. J med microbiol. 2011;60(4):477-80. [DOI:10.1099/jmm.0.026633-0]
14. Diab-Elschahawi M, Forstner C, Hagen F, Meis JF, Lassnig AM, Presterl E, et al. Microsatellite genotyping clarified conspicuous accumulation of Candida parapsilosis at a cardiothoracic surgery intensive care unit. J clin Microbiol. 2012;50(11):3422-6. [DOI:10.1128/JCM.01179-12]
15. Khodavaisy S, Badali H, Rezaie S, Nabili M, Moghadam KG, Afhami S, et al. Genotyping of clinical and environmental Aspergillus flavus isolates from Iran using microsatellites. Mycoses. 2016;59(4):220-5. [DOI:10.1111/myc.12451]
16. Cantón E, Pemán J, Quindós G, Eraso E, Miranda-Zapico I, Álvarez M, et al. Prospective multicenter study of the epidemiology, molecular identification, and antifungal susceptibility of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis isolated from patients with candidemia. Antimicrob Agents Chemother. 2011;55(12):5590-6. [DOI:10.1128/AAC.00466-11]
17. De Valk HA, Meis JF, Curfs IM, Muehlethaler K, Mouton JW, Klaassen CH. Use of a novel panel of nine short tandem repeats for exact and high-resolution fingerprinting of Aspergillus fumigatus isolates. J Clin Microbiol. 2005;43(8):4112-20. [DOI:10.1128/JCM.43.8.4112-4120.2005]
18. Arendrup MC. Epidemiology of invasive candidiasis. Curr Opin Crit Care. 2010;16(5):445-52. [DOI:10.1097/MCC.0b013e32833e84d2]
19. Lockhart SR, Iqbal N, Cleveland AA, Farley MM, Harrison LH, Bolden CB, et al. Species identification and antifungal susceptibility testing of Candida bloodstream isolates from population-based surveillance studies in two US cities from 2008 to 2011. J Clin Microbiol. 2012;50(11):3435-42 [DOI:10.1128/JCM.01283-12]
20. Pfaller MA, Jones RN, Castanheira M. Regional data analysis of Candida non‐albicans strains collected in United States medical sites over a 6‐year period, 2006–2011. Mycoses. 2014;57(10):602-11. [DOI:10.1111/myc.12206]
21. Trofa D, Gácser A, Nosanchuk JD. Candida parapsilosis, an emerging fungal pathogen. Clin Microbiol Rev. 2008;21(4):606-25. [DOI:10.1128/CMR.00013-08]
22. Khodavaisy S, Alialy M, Mahdavi Omran S, Habibi MR, Amri P, Monadi M, et al. The study on fungal colonization of respiratory tract in patients admitted to intensive care units of sari and babol hospitals. Med J mashhad Univ Med Sci. 2011;54(3):177-84.
23. Lotfali E, Kordbacheh P, Mirhendi H, Zaini F, Ghajari A, Mohammadi R, et al. Antifungal susceptibility analysis of clinical isolates of Candida parapsilosis in Iran. Iran j public health. 2016;45(3):322-8.
24. Mohammadi R, Badiee P, Badali H, Abastabar M, Safa AH, Hadipour M, et al. Use of restriction fragment length polymorphism to identify Candida species, related to onychomycosis. Adv Biomed Res. 2015;4:95. [DOI:10.4103/2277-9175.156659]
25. Mohammadi R, Mirhendi H, Hedayati MT, Badali H. Caspofungin-Non-Susceptible Candida orthopsilosis Isolated from Onychomycosis in Iran. Iran j public health. 2017;46(2):235-41.
26. Chao Q-T, Lee T-F, Teng S-H, Peng L-Y, Chen P-H, Teng L-J, et al. Comparison of the accuracy of two conventional phenotypic methods and two MALDI-TOF MS systems with that of DNA sequencing analysis for correctly identifying clinically encountered yeasts. PLoS One. 2014;9(10):e109376. [DOI:10.1371/journal.pone.0109376]
27. del Pilar Vercher M, Martínez JMG, Cantón E, Pemán J, García MMG, Gómez EV, et al. Differentiation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis by specific PCR amplification of the RPS0 intron.Int J Med Microbiol. 2011;301(6):531-5. [DOI:10.1016/j.ijmm.2011.02.001]
28. Souza ACR, Ferreira RC, Gonçalves SS, Quindós G, Eraso E, Bizerra FC, et al. Accurate identification of Candida parapsilosis (sensu lato) by use of mitochondrial DNA and real-time PCR. J Clin Microbiol. 2012;50(7):2310-4. [DOI:10.1128/JCM.00303-12]
29. De Carolis, Hensgens LA, Vella A, Posteraro B, Sanguinetti M, Senesi S, et al. Identification and typing of the Candida parapsilosis complex: MALDI-TOF MS vs. AFLP. Med Mycol. 2014;52(2):123-30. [DOI:10.1093/mmy/myt009]
30. Tavanti A, Hensgens LA, Ghelardi E, Campa M, Senesi S. Genotyping of Candida orthopsilosis clinical isolates by amplification fragment length polymorphism reveals genetic diversity among independent isolates and strain maintenance within patients. J Clin Microbiol. 2007;45(5):1455-62. [DOI:10.1128/JCM.00243-07]
31. Van Asbeck EC, Clemons KV, Markham AN, Stevens DA. Correlation of restriction fragment length polymorphism genotyping with internal transcribed spacer sequence, randomly amplified polymorphic DNA and multilocus sequence groupings for Candida parapsilosis. Mycoses. 2009;52(6):493-8. [DOI:10.1111/j.1439-0507.2008.01649.x]
32. Foulet F, Nicolas N, Eloy O, Botterel F, Gantier J-C, Costa J-M, et al. Microsatellite marker analysis as a typing system for Candida glabrata. J Clin microbiol. 2005;43(9):4574-9. [DOI:10.1128/JCM.43.9.4574-4579.2005]
33. Hennequin C, Thierry A, Richard G, Lecointre G, Nguyen H, Gaillardin C, et al. Microsatellite typing as a new tool for identification of Saccharomyces cerevisiae strains. J Clin Microbiol. 2001;39(2):551-9. [DOI:10.1128/JCM.39.2.551-559.2001]
34. Botterel F, Desterke C, Costa C, Bretagne S. Analysis of microsatellite markers of Candida albicans used for rapid typing. J clin Microbiol. 2001;39(11):4076-81. [DOI:10.1128/JCM.39.11.4076-4081.2001]
35. Lasker BA, Butler G, Lott TJ. Molecular genotyping of Candida parapsilosis group I clinical isolates by analysis of polymorphic microsatellite markers. J clin Microbiol. 2006;44(3):750-9. [DOI:10.1128/JCM.44.3.750-759.2006]
36. Sabino R, Sampaio P, Rosado L, Stevens DA, Clemons KV, Pais C. New polymorphic microsatellite markers able to distinguish among Candida parapsilosis sensu stricto isolates. J Clin microbiol. 2010;48(5):1677-82. [DOI:10.1128/JCM.02151-09]

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