Comparison of aglycon and glycosidic saponin extracts of Cyclamen coum tuber against Candida spp.

Authors

1 Department of Plant Sciences, Faculty of Biological Sciences, Alzahra University, Tehran, Iran

2 Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran

Abstract

Background and Purpose: Candidiasis, an important fungal infection, is considered the fourth most common nosocomial
blood stream infection. Nowadays, because of increased fungal resistance to antibiotics, the use of herbal medicine has
gained particular attention. Cyclamen species are medicinal plants containing triterpenoid saponins, which are shown to
have antimicrobial properties.
Materials and Methods: Three species of Candida including C. albicans 10231, C. tropicalis 0750, and C. krusei
and nine clinical samples were cultured on Sabouraud dextrose agar. Active substances of the tubers were extracted by
fractionation method. Susceptibility of Candida to Cyclamen coum tuber extracts was evaluated via minimum inhibitory
concentration (MIC) and minimum fungicidal concentration (MFC).
Results: Our results demonstrated that ethyl acetate extract had no inhibitory effect on Candida strains, whereas the
aqueous and n-butanolic extracts showed considerable activity. MIC and MFC of these extracts varied within the
range of 2-32 μg/mL of saponin for different Candida samples. Aglyconic aqueous phase of the extract had the most
effective anticandida activity. Glycosidic and aglyconic aqueous extracts were less active on C. albicans strains and C.
tropicalis, respectively.
Conclusion: Tuber extract of Cyclamen was rich in triterpenoid saponins and had antifungal effect. Sugar chain structure,
as well as type and concentration of the aglycones were effective in this activity.

Keywords


1. Saad A, Fadli M, Bouaziz M, Benharref A, Mezrioui NE, Hassani L. Anticandidal activity of the essential oils of Thymus maroccanus and Thymus broussonetii and their synergism with amphotericin B and fluconazole. Phytomedicine. 2010; 17(13):1057-60.
2. Sortino M, Cechinel Filho V, Correˆab R, Zacchino S. N-Phenyl and N-phenylalkyl-maleimides acting against Candida spp.: time-to-kill, stability, interaction with maleamic acids. Bioorg Med Chem. 2008;
16(1):560–8.
3. Bertout S, Dunyach C, Drakulovski P, Reynes J, Mallie M. Comparison of the Sensititre Yeast One® dilution method with the Clinical Laboratory Standards Institute (CLSI) M27-A3 microbroth dilution reference method for determining MIC of eight antifungal agents on 102 yeast strains. Pathol Biol. 2011; 59(1):48–51.
4. Shreaz S, Bhatia R, Khan N, Muralidhar S, Seemi FB, Nikhat M, et al. Spice oil cinnamaldehyde exhibits potent anticandidal activity against fluconazole resistant clinical isolates. Fitoterapia. 2011; 82(7):1012–20.
5. Stuardo M, San Mart´ın R. Antifungal properties of quinoa (Chenopodium quinoa Willd) alkali treated saponins against Botrytis cinerea. Ind Crops Prod. 2008; 27(3):296-302.
6. Marzouk B, Marzouk Z, Décor R, Edziri H, Haloui E, Fenina N, et al. Antibacterial and anticandidal screening of Tunisian Citrullus colocynthis Schrad. from Medenine. J Ethnopharmacol. 2009; 125(2):344 9.
7. Mert-Türk F. Saponins versus plant fungal pathogens. J Cell Mol Biol. 2006; 5:13-7.
8. Hassan SM, Haq AU, Byrd JA, Berhow MA, Cartwright AL, Bailey CA. Haemolytic and antimicrobial activities of saponin-rich extracts from guar meal. Food Chem. 2010; 119(2):600–5.
9. Lanzotti V, Romano A, Lanzuise S, Bonanomi G, Scala F. Antifungal saponins from bulbs of white onion, Allium cepa L. Phytochemistry. 2012; 74:133–9.
10. Calis I, Yuruker A, Tanker N, Wright AD, Sticher O. Triterpene saponins from Cyclamen coum var. coum. Planta Med.1997; 63(2):166-70.
11. Altunkeyik H, Gülcemal D, Masullo M, Alankus- Caliskana O, Piacenteb S, Karayildirim T. Triterpene saponins from Cyclamen hederifoliun. Phytochemistry. 2012; 73(1):127-33.
12. Ma L, Gu YC, Lou JG, Wang JS, Huang XF, Kong LY. Triterpenoid saponins from Dianthus versicolor. J Nat Prod. 2009; 72(4):640-4.
13. Turkoglu A, Duru ME, Mercan N, Kivrak I, Gezer K. Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill. Food Chem. 2007; 101(1):267–73.
14. Damke E, Tsuzuki JK, Cortez DA, Ferreira IC, Bertoni TA, Batista MR, et al. In vivo activity of Sapindus saponaria against azole-susceptible and-resistant human vaginal Candida species. BMC Complement Altern Med. 2011; 11:35-43.
15. Wu J, Lin L, Chau FT. Ultrasound-assisted extraction of ginseng saponins from ginseng roots and cultured ginseng cells. Ultrason Sonochem. 2001; 8(4):347-52.
16. Marrinova D, Ribarov F, Atanassova M. Total phenolics and total flavonoids in Bolgharian fruits and vegetables. J Univ Chem Technol Metall. 2005; 40(3):255-60.
17. IBM Corp, Released. IBM SPSS statistics for windows. Armonk, NY: IBM Corp; 2010.
18. Gyawali R, Ibrahim SA. Natural products as antimicrobial agents. Food Control. 2014; 46:412-29.
19. Deeptanshu S. Saponin and its biological actions with special reference to Fenugreek. [Doctoral Thesis]. Karnataka, India: University of Mysore; 2009.
20. Sparg SG, Light ME, van Staden J. Biological activities and distribution of plant saponins. J Ethnopharmacol. 2004; 94(2-3):219–43.
21. Pawar HA, Shenoy AV, Narawade PD, Soni PY, Shanbhag PP, Rajal PA. Preservatives from Nature: a review. Int J Pharm Phytopharmacol Res. 2011; 1(2):78-88.
Volume 2, Issue 2
June 2016
Pages 40-44
  • Receive Date: 09 July 2019
  • Revise Date: 08 October 2020
  • Accept Date: 09 July 2019
  • Publish Date: 01 June 2016