Department of Mycology, School Of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Department of Dermatology, School Of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Department of Bacteriology, School Of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Student of MD, School Of Medicine, Alborz University of Medical Sciences, Karaj, Iran
Biology Researcher , Department of Food Science and Technology, Faculty of Agriculture, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Department of Pathobiology, Food Microbiology Division, School of Public Health, Tehran University of Medical Sciences, Tehran, Ira
Background and Purpose: Seborrheic dermatitis is a chronic and recurrent superficial dermatitis in which Malassezia species play an important role. There are different Malassezia species, which have been recently reported to be resistant to common antifungals. Natural sources can be useful alternatives to reduce the emergence of this resistance. Kombucha tea is believed to have potential antimicrobial properties. Regarding this, the present study aimed to investigate theantifungal activity of Kombucha tea ethyl acetate fraction (KEAF) against Malassezia species obtained from the
patients with seborrheic dermatitis.
Materials and Methods: A total of 23 clinical isolates were identified by direct microscopic examination and Tween assimilation, and then confirmed by DNA sequencing of ITS regions for Malassezia species. Kombucha tea was fractionated using ethyl acetate (1:2 v/v). The minimum inhibitory concentration (MIC) microdilution assay was used to evaluate the anti-Malssezia activity of KEAF at three concentrations of 10, 40, and 80 mg/mL.
Results: The results of the DNA sequence analysis indicated that M. furfur (39.13%) was the predominant species,followed by M. globosa (30.43%), M. sloofie (13.04%), M. sympodialis (13.04%), and M. restricta (4.34%), respectively. Furthermore, KEAF showed inhibitory activity against Malassezia species. Accordingly, KEAF had the lowest and highest MIC value against M. sloofie and M. restricta, respectively. Moreover, the inhibitory effect of the extract was equivalent to that of ketoconazole at 4.8 µg/mL.
Conclusion: The findings of the current study highlighted the antifungal properties of KEAF. Therefore, this extract can be promoted as complementary medicine for the treatment of the infections caused by Malassezia.