DNA sequence analysis of keratinophilic fungi isolated from livestock stables in the East of Iran

Document Type : Short Communication

Authors

1 Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

2 Cutaneous Leishmaniasis Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

3 Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Background and Purpose: Keratinophilic fungi play an important role in the decomposition of keratinous substances in nature. This capacity induces dermatomycosis in both humans and livestock. The soil of livestock stables can be a reservoir of keratinophilic fungi. Therefore, the present study was conducted to isolate and identify keratinophilic fungi in the soil of the livestock stables located in Qayen, South Khorasan Province, Iran.
Materials and Methods: This study was conducted on 62 soil samples collected from livestock stables. The samples were cultured by means of hair bait technique (HBT). The identification of the isolates was performed based on their morphological characteristics and then confirmed by polymerase chain reaction and sequencing of the ITS regions of ribosomal DNA.
Results: A total of 118 isolates of 7 species from 5 genera were identified. Aphanoascus verrucosu (n=70, 59.36%) was detected as the dominant keratinophilic fungus, followed by Arthroderma quadrifidum (n=13, 11.01%), Aphanoascus terreus (n=12, 10.16%), Acremonium (n=12, 10.16%), Arthroderma gertleri (n=5, 4.23%), Fusarium equiseti (n=3, 2.54%), and Uncinocarpus reesii (n=3, 2.54%).
Conclusion: Different keratinophilic fungi were isolated from the soil of livestock stables; however, Aphanoascus verrucosu was found to be the dominant species.
 

Keywords

References

1. Dehghan P, Yousefi Jalali S, Chadeganipour M. Frequency distribution of Keratinophilic dermatophyte fungi from the soil of different zones in Isfahan using morphological and molecular methods. Adv Biomed Res. 2019; 8:38
2. Vanbreuseghem R. Biological technique for the isolation of dermatophytes from the soil. Ann Soc Belge Med Trop. 1952; 32(2):173-8.
3. Pakshir K, Ghiasi MR, Zomorodian K, Gharavi AR. Isolation and molecular identification of keratinophilic fungi from public parks soil in Shiraz, Iran. Biomed Res Int. 2013; 2013:619576.
4. Kushwaha RK, Guarro J. Biology of dermatophytes and other keratinophilic fungi. Spain: Revista Iberoamericana de Micologia; 2000.
5. Zaki SM, Mikami Y, El-Din AA, Youssef YA. Keratinophilic fungi recovered from muddy soil in Cairo vicinities, Egypt. Mycopathologia. 2005; 160(3):245-51.
6. McAleer R. Investigation of keratinophilic fungi from soils in Western Australia a preliminary survey. Mycopathologia. 1980; 72(3):155-65.
7. Altayyar IA, Osman NA, Elbreki MF, Ibrahim H, Aboalasad A, Barkah A, et al. Isolation and identification of soil keratinophilic fungi from different area in south of Libya. Int J Appl Med Biol Res. 2016; 1(1):27-32.
8. Deshmukh SK, Mandeel QA, Verekar SA. Keratinophilic fungi from selected soils of Bahrain. Mycopathologia. 2008; 165(3):143-7.
9. Pontes ZB, Oliveira AC, Guerra FQ, Pontes LR, Santos JP.Distribution of dermatophytes from soils of urban and rural areas of cities of Paraiba state, Brazil. Rev Inst Med Trop Sao Paulo. 2013; 55(6):377-83.
10. Lee MJ, Park JS, Chung H, Jun JB, Bang YJ. Distribution of soil keratinophilic fungi isolated in summer beaches of the East Sea in Korea. Korea J Med Mycol. 2011; 16:44-50.
11. Moalaei H, Zeyni F, Mahmoudi M, Hashemi J, Pit M. The survey and identification of keratinophilic fungi in dry-farming soil samples from South and Razavi Khorasan provinces in Iran. J Sabzevar Univ Med Sci. 2006; 13(2):64-73.
12. Shokohi T, Hedayati M, Bakhshi H. Isolation of fungi and aerobic actinomycetes from surface soil in Sari. J Kermanshah Univ Med Sci. 2005; 8:25-32.
13. Aghamirian MR, Ghiasian SA. The prevalence of fungi in soil of Qazvin, Iran. Jundishapur J Microbiol. 2013; 6(1):76-9.
14. Rezaei-Matehkolaei A, Jahangiri A, Mahmoudabadi AZ, Najafzadeh MJ, Nouripour-Sisakht S, Makimura K. Morpho-molecular characterization of soil inhabitant dermatophytes from Ahvaz, Southwest of Iran, a high occurrence of Microsporum fulvum. Mycopathologia. 2017; 182(7-8):691-9.
15. Rezaei-Matehkolaei A, Makimura K, de Hoog GS, Shidfar MR, Satoh K, Najafzadeh MJ, et al. Multilocus differentiation of the related dermatophytes Microsporum canis, Microsporum ferrugineum and Microsporum audouinii. J Med Microbiol. 2012; 61(Pt 1):57-63.
16. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990; 215(3):403-10.
17. Rizwana H, Abdulaziz Al Hazzani A, Siddiqui I. Prevalence of dermatophytes and other keratinophilic fungi from soils of Public Parks and Playgrounds of Riyadh, Saudi Arabia. J Anim Plant Sci. 2012; 22(4):948-53.
18. Naseri A, Fata A, Khosravi AR. Tinea capitis due to Microsporum vanbreuseghemii: report of two cases. Mycopathologia. 2012; 174(1):77-80.
19. Deshmukh SK, Verekar SA. Incidence of keratinophilic fungi from selected soils of Vidarbha region of Maharashtra State, India. J Mycol. 2014; 2014:148970.
20. De Hoog GS, Guarro J, Gene J, Figure MJ. Atlas of clinical fungi. 2nd ed. Tarragona, Spain: Rovira i Virgili University; 2000.
21. Rippon JW, Lee FC, McMillen S. Dermatophyte infection caused by Aphanoascus fulvescens. Arch Dermatol. 1970; 102(5):552-5.
22. Davari M, van Diepeningen AD, Babai-Ahari A, Arzanlou M, Najafzadeh MJ, van der Lee TA, et al. Rapid identification of Fusarium graminearum species complex using Rolling Circle Amplification (RCA). J Microbiol Methods. 2012; 89(1):63-70.
23. Arnoni MV, Paula CR, Auler ME, Simões CC, Nakano S, Szeszs MW, et al. Infections caused by fusarium species in pediatric cancer patients and review of published literature. Mycopathologia. 2018; 183(6):941-9.
24. Covington M, Gao J, Abdulla F, Rosić VP. Disseminated Fusarium infections in acute lymphoblastic leukemia, Serbian J Dermatol Venereol. 2018; 10(2):43-6.
25. Haghani I, Shams-Ghahfarokhi M, Dalimi Asl A, Shokohi T, Hedayati MT. Molecular identification and antifungal susceptibility of clinical fungal isolates from onychomycosis (uncommon and emerging species). Mycoses. 2019; 62(2):128-43.
Current Medical Mycology
Volume 6, Issue 3
September 2020
Pages 57-60

Files

Share

How to cite

Statistics