Epidemiological characterization of pityriasis versicolor and distribution of Malassezia species among students in Hai Phong city, Vietnam

Document Type : Original Articles

Authors

1 Department of Genetics and Cytogenetics, Institute for Military Medical Research, Military Medical University, Ha Noi, Vietnam

2 Department of Medical Parasitology, Hai Phong University of Medicine and Pharmacy, Hai Phong, Vietnam

3 Department of Clinical Parasitology, National Institute of Malaria Parasitology and Entomology, Ha Noi, Vietnam

4 Department of Medical Biology and Genetics, Military Medical University, Ha Noi, Vietnam

5 Ha Noi University of Public Health, Ha Noi, Vietnam

6 Department of Medical Parasitology, Military Medical University, Ha Noi, Vietnam

Abstract

Background and Purpose: Pityriasis versicolor (PV) is a common fungal skin infection caused by Malassezia species. Previous studies have shown that the prevalence of PV is influenced by geographic factors. The aim of the current study was to find the epidemiological characteristics of PV and distribution of Malassezia species in the secondary school students living in Hai Phong city, Vietnam.
Materials and Methods: This study was conducted on 1357 students within the age range of 10 - 16 years selected from four secondary schools in Hai Phong city. The students were screened for PV skin lesions from August 2016 to December 2017. The isolates of Malassezia from PV patients were analyzed by performing direct microscopy and culturing on modified Dixon agar plates, containing gentamicin, at 32oC for 7 days. In the next stage, the fungal strains obtained from patients with positive fungal cultures were identified using the CHROMagarTM Malassezia medium, polymerase chain reaction-restriction fragment length polymorphism techniques, and D1/D2 rDNA genome sequencing.
Results: Pityriasis versicolor was diagnosed in 305 (22.48%) students and confirmed by clinical appearance and direct examination. A total of 293 (96.07%) samples grew on modified Dixon agar. With regard to demographic characteristics, 50.49% of the PV cases were female, and 57.38% of cases resided in urban areas. Furthermore, 88.52% of the subjects had the illness duration of more than 6 months. Hypopigmented and erythematous skin lesions were also observed in the research participants, with hypopigmentation being the most frequent condition (97.05%). Most of the Malassezia fungal strains were isolated from the back (39.56%), face (23.99%), and chest (16.51%). Malassezia furfur and M. japonica accounted for PV in 96.25% and 3.75% of the cases, respectively. Furthermore, Malassezia furfur was distributed in both rural and urban areas, while M. japonica was found only in the urban areas.
Conclusion: The findings of the present study were indicative of the high prevalence of Malassezia yeasts, mostly M. furfur, among the students in Hai Phong city, Vietnam
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Keywords


1. Zeinali E, Sadeghi G, Yazdinia F, Shams-Ghahfarokhi M, Razzaghi-Abyaneh M. Clinical and epidemiological features of the genus Malassezia in Iran. Iran J microbiol. 2014; 6(5):354-60.
2. Oh BH, Song YC, Lee YW, Choe YB, Ahn KJ. Comparison of Nested PCR and RFLP for Identification and Classification of Malassezia Yeasts from Healthy Human Skin. Ann Dermatol. 2009; 21(4):352-7.
3. Affes M, Ben Salah S, Makni F, Sellami H, Ayadi A. Molecular identification of Malassezia species isolated from dermatitis affections. Mycoses. 2009; 52(3):251-6.
4. Honnavar P, Chakrabarti A, Dogra S, Handa S, Rudramurthy SM. Phenotypic and molecular characterization of Malassezia japonica isolated from psoriasis vulgaris patients. J Med Microbiol. 2015; 64(Pt 3):232-6.
5. Gaitanis G, Magiatis P, Hantschke M, Bassukas ID, Velegraki A. The Malassezia genus in skin and systemic diseases. Clin Microbiol Rev. 2012; 25(1):106-41.
6. Moallaei H, Namazi MJ, Bouchara JP, Pourhammed S. Malassezia species in students from universities of Sabzevar, Northeastern Iran. J Mycol Med. 2018; 28(1):70-5.
7. Ilahi A, Hadrich I, Neji S, Trabelsi H, Makni F, Ayadi A. Real-Time PCR Identification of Six Malassezia Species. Curr Microbiol. 2017; 74(6):671-7.
8. Castella G, Coutinho SD, Cabanes FJ. Phylogenetic relationships of Malassezia species based on multilocus sequence analysis. Med Mycol. 2014; 52(1):99-105.
9. Rudramurthy SM, Honnavar P, Dogra S, Yegneswaran PP, Handa S, Chakrabarti A. Association of Malassezia species with dandruff. Indian J Med Res. 2014; 139(3):431-7.
10. Zhao Y, Li L, Wang JJ, Kang KF, Zhang QQ. Cutaneous malasseziasis: four case reports of atypical dermatitis and onychomycosis caused by Malassezia. Int J Dermatol. 2010; 49(2):141-5.
11. Iatta R, Battista M, Miragliotta G, Boekhout T, Otranto D, Cafarchia C. Blood culture procedures and diagnosis of Malassezia furfur bloodstream infections: Strength and weakness. Med Mycol. 2018; 56(7):828-33.
12. Benito-Leon J, Laurence M. Malassezia in the central nervous system and multiple sclerosis. Infection. 2019; 47(1):135-6.
13. Ibekwe PU, Ogunbiyi AO, Besch R, Ruzicka T, Sardy M. The spectrum of Malassezia species isolated from students with pityriasis vesicolor in Nigeria. Mycoses. 2015; 58(4):203-8.
14. Vuran E, Karaarslan A, Karasartova D, Turegun B, Sahin F. Identification of Malassezia species from pityriasis versicolor lesions with a new multiplex PCR method. Mycopathologia. 2014; 177(1-2):41-9.
15. Lian CH, Shen LL, Gao QY, Jiang M, Zhao ZJ, Zhao JJ. Identification of Malassezia species in the facial lesions of Chinese seborrhoeic dermatitis patients based on DNA
sequencing. Mycoses. 2014; 57(12):759-64.
16. Krisanty RI, Bramono K, Made Wisnu I. Identification of Malassezia species from pityriasis versicolor in Indonesia and its relationship with clinical characteristics. Mycoses. 2009; 52(3):257-62.
17. Cam VT, Van TN, Hau KT, Huu DL, Minh PPT, Huu SN, et al. Distribution of Malassezia Species from Scales of Patient with Pityriasis Versicolor by Culture in Vietnam. Open access Maced J Med Sci. 2019; 7(2):184-6.
18. Giusiano G, Sosa Mde L, Rojas F, Vanacore ST, Mangiaterra M. Prevalence of Malassezia species in pityriasis versicolor lesions in northeast Argentina. Rev Iberoam Micol. 2010; 27(2):71-4.
19. Xie Z, Ran Y, Zhang H, Zhang M, Wan H, Li C. An analysis of the Malassezia species distribution in the skin of patients with pityriasis versicolor in Chengdu, China. Sci World J. 2014; 2014:182596.
20. Oke OO, Onayemi O, Olasode OA, Omisore AG, Oninla OA. The Prevalence and Pattern of Superficial Fungal Infections among School Children in Ile-Ife, South-Western Nigeria. Dermatol Res Pract. 2014; 2014:842917.
21. Chaudhary R, Singh S, Banerjee T, Tilak R. Prevalence of different Malassezia species in pityriasis versicolor in central India. Indian J Dermatol Venereol Leprol. 2010; 76(2):159-64.
22. Rasi A, Naderi R, Behzadi AH, Falahati M, Farehyar S, Honarbakhsh Y, et al. Malassezia yeast species isolated from Iranian patients with pityriasis versicolor in a prospective study. Mycoses. 2010; 53(4):350-5.
23. Tarazooie B, Kordbacheh P, Zaini F, Zomorodian K, Saadat F, Zeraati H, et al. Study of the distribution of Malassezia species in patients with pityriasis versicolor and healthy individuals in Tehran, Iran. BMC Dermatol. 2004; 4:5.
24. Sharma A, Rabha D, Choraria S, Hazarika D, Ahmed G, Hazarika NK. Clinicomycological profile of pityriasis versicolor in Assam. Indian J Pathol & Microbiol. 2016; 59(2):159-65.
25. Shah A, Koticha A, Ubale M, Wanjare S, Mehta P, Khopkar U. Identification and speciation of Malassezia in patients clinically suspected of having pityriasis versicolor. Indian J Dermatol. 2013; 58(3):239.
26. Lee YW, Byun HJ, Kim BJ, Kim DH, Lim YY, Lee JW et al. Distribution of Malassezia species on the scalp in Korean seborrheic dermatitis patients. Ann Dermatol. 2011; 23(2):156-61.
27. Awad AK, Al-Ezzy AI, Jameel GH. Phenotypic Identification and Molecular Characterization of Malassezia spp. isolated from Pityriasis versicolor patients with special emphasis to risk factors in Diyala province, Iraq. Open access Maced J Med Sci. 2019; 7(5):707-14.
28. Saxena R, Mittal P, Clavaud C, Dhakan DB, Hegde P, Veeranagaiah MM, et al. Comparison of healthy and dandruff scalp microbiome reveals the role of commensals in Scalp Health. Front Cell Infect Microbiol. 2018; 8:346.
Volume 6, Issue 2
June 2020
Pages 11-17
  • Receive Date: 06 November 2019
  • Revise Date: 20 May 2020
  • Accept Date: 26 May 2020
  • First Publish Date: 01 June 2020