Effects of Bunium persicum essential oil on the reduction of spore germination, growth, and expression of FUM1 and FUM14 genes in Fusarium verticillioides isolates

Document Type : Original Articles

Authors

1 Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranUniversity of Tehran

2 Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran

10.18502/CMM.7.2.7033

Abstract

Background and Purpose: Black Cumin of Kerman (Bunium persicum) is an Iranian plant that is commonly used as an antispasmodic, carminative, and antimicrobial substance. The present study aimed to assess different components of the essence of B.persicum and its effect on antifungal activity, spore germination inhibition, and expressions of FUM1 and FUM14 genes in Fusarium verticillioides strains.
Materials and Methods: The essence was extracted by hydrodistillation and analyzed through gas chromatography-mass spectroscopy. A broth microdilution method was used for the determination of the minimum inhibitory concentration (MIC). In addition, the expression of FUM1 and FUM14 genes of toxigenic F. verticillioides was assessed by
using the real-time polymerase chain reaction (RT-PCR) technique.
Results: Based on the findings, most of the essence consisted of γ-terpinene (15.56%),propanal, and 2-methyl-3-phenyl (14.18%). The oil showed a good antifungal activity(mean MIC value: 2556.8 μg/ml) as well as the inhibition of spore germination and mycelial growth (P<0.05). The RT-PCR demonstrated that the expression levels of FUM1 and FUM14 of B. persicum-treated F. verticillioides were 0.43 and 0.53 folds ower than the control samples, respectively.
Conclusion: These findings revealed that the essential oil of B. persicum has different components responsible for the inhibition of mycelial growth and spore germination of F. verticillioides as well as reduction of expressions of FUM1 and FUM14 genes involving fumonisin production.


 

Highlights

1. Chen Y, HuangTT, Chen CJ, Hou YP, Zhang AF, Wang WX, et
al. Sensitivity of Fusarium verticillioides isolates from rice to a
novel cyanoacrylate fungicide. Crop Prot. 2012; 39(2):106-9.
2. Thippeswamy S, Mohana DC, Abhishek RU, Manjunath K.
Effect of plant extracts on inhibition of Fusarium verticillioides
growth and its toxin fumonisin B1 production. J Agric Sci
Technol. 2003; 9(4):889-900.
3. Palacios SA, Susca A, Haidukowski M, Stea G, Cendoya E,
Ramírez ML, et al. Genetic variability and fumonisin production
by Fusarium proliferatum isolated from durum wheat grains in
Argentina. Int J Food Microbiol. 2015; 201(1):35-41.
4. Guo Z, Doll K, Dastjerdi R, Karlovsky P, Dehne HW,
Altincicek B. Effect of fungal colonization of wheat grains with
Fusarium spp. on food choice weight gain and mortality of meal
beetle larvae (Tenebrio molitor). PLoS One. 2014;
9(6):e100112.
5. Panda P, Aiko V, Mehta A. Effect of aqueous extracts of Mentha
arvensis (mint) and Piper betle (betel) on growth and citrinin
production from toxigenic Penicillium citrinum. J Food Sci
Technol. 2015; 52(6):3466-74.
6. Divband K, Shokri H, Khosravi AR. Down-regulatory effect of
Thymus vulgaris L. on growth and Tri4 gene expression in
Fusarium oxysporum strains. Microb Pathog. 2017; 104(1):1-5.
7. Azizi M, Davareenejad G, Bos R, Woerdenbag HJ, Kayser O.
Essential oil content and constituents of Black Zira (Bunium
persicum [Boiss.] B. Fedtsch.) from Iran during field cultivation
(Domestication). J Essen Oil Res. 2009; 21(1):78-82.
8. Oroojalian F, Kasra-Kermanshahi R, Azizi M, Bassami MR.
Phytochemical composition of the essential oils from three
Apiaceae species and their antibacterial effects on food-borne
pathogens. Food Chem. 2010; 120(3):765-70.
9. Sekine T, Sugano M, Majid A, Fujii Y. Antifungal effects of
volatile compounds from Black Zira (Bunium persicum) and
other spices and herbs. J Chem Ecol. 2007; 33(11):2123-32.
10. Council of Europe. Methods of pharmacognosy. European
pharmacopoeia. 3rd ed. Strasbourg: European Department for
the Quality of Medicines; 1997. P. 121-2.
11. Clinical and Laboratory Standards Institute (CLSI). Reference
method for broth dilution antifungal susceptibility testing of
filamentous fungi; approved standard. 2nd ed. Wayne, PA:
Clinical and Laboratory Standards Institute; 2008.
12. Mohammadi A, Hashemi M, Hosseini SM. Comparison of
antifungal activities of various essential oils on the Phytophthora
drechsleri, the causal agent of fruit decay. Iran J Microbiol.
2015; 7(1):31-7.
13. Kalagatur NK, Kamasani JR, Mudili V, Krishna K, Chauhan
OP, Sreepathi MH. Effect of high pressure processing on growth
and mycotoxin production of Fusarium graminearum in maize.
Food Biosci. 2018; 21(1):53-9.
14. Rocha LO, Barroso VM, Andrade LJ, Pereira GHA, FerreiraCastro FL, Duarte AP, et al. FUM gene expression profile and
fumonisin production by Fusarium verticillioides inoculated in
Bt and non-Bt maize. Front Microbiol. 2016; 6:1503.
15. Khaledi N, Hassani F. Antifungal activity of Bunium persicum
essential oil and its constituents on growth and pathogenesis of
Colletotrichum lindemuthianum. J Plant Protect Res. 2018;
58(4):431-41.
16. Sharafati Chaleshtori F, Saholi M, Sharafati Chaleshtori R.
Chemical composition, antioxidant and antibacterial activity of
Bunium persicum, Eucalyptus globulus, and Rose Water on  multidrug-resistant Listeria species. J Evid Based Integ Med.
2018; 23(1):1-7.
17. Shahsavari N, Barzegar M, Sahari MA, Naghdibadi H.
Antioxidant activity and chemical characterization of essential
oil of Bunium persicum. Plant Foods Human Nutr. 2008;
63(4):183-8.
18. Azimzadeh M, Amiri R, Assareh MH, Bihamta MR, Forootan
M. Genetic diversity of Iranian Bunium persicum germplasm by
morphological markers and essential oil components. J Med
Plant Res. 2012; 6(7):1119-29.
19. Naeini A, Ziglari T, Shokri H, Khosravi AR. Assessment of
growth-inhibiting effect of some plant essential oils on different
Fusarium isolates. J Mycol Med. 2010; 20(2):174-8.
20. Behtoei H, Amini J, Javadi T, Sadeghi A. Composition and in
vitro antifungal activity of Bunium persicum, Carum copticum
and Cinnamomum zeylanicum essential oils. J Med Plants Res.
2012; 6(37):5069-76.
21. Khaledi N, Taheri P, Arighi S. Antifungal activity of various
essential oils against Rhizoctonia solani and Macrophomina
phaseolina as major bean pathogens. J Appl Microbiol. 2014;
118(3):704-17.
22. Proctor RH, Van Hove F, Susca A, Stea G, Busman M, Van der
Lee T, et al. Birth, death and horizontal gene transfer of the
fumonisin byosinthetic gene cluster during the evolutionary
diversification of Fusarium. Mol Microbiol. 2013; 90(2):290-306.
23. Fanelli F, Schmidt-Heydt M, Haidukowski M, Geisen R,
Logrieco A, Mulè G. Influence of light on growth, fumonisin
biosynthesis and FUM1 gene expression by Fusarium
proliferatum. Int J Food Microbiol. 2012; 153(1-2):148-53.
24. Lazzaro I, Susca A, Mule G, Ritieni A, Ferracane R, Marocco A.
Effects of temperature and water activity on FUM2 and FUM21
gene expression and fumonisin B production in Fusarium
verticillioides. Eur J Plant Pathol. 2012; 134(4):685-95.
25. Lozano-Ojalvo D, Rodríguez A, Bernáldez V, Córdoba JJ,
Rodríguez M. Influence of temperature and substrate conditions
on the omt-1 gene expression of Aspergillus parasiticus in
relation to its aflatoxin production. Int J Food Microbiol. 2013;
166(2):263-9.
26. Lopez-Errasquın E, Vazquez C, Jimenez M, Gonzalez-Jaen MT.
Real-time RT-PCR assay to quantify the expression of FUM1
and FUM19 genes from the fumonisin-producing Fusarium
verticillioides. J Microbiol Methods. 2007; 68(2):312-7.
27. Khosravi AR, Shokri H, Mokhtari AR. Efficacy of Cuminum
cyminum essential oil on FUM1 gene expression of fumonisinproducing Fusarium verticillioides strains. Avicenna J
Phytomed. 2015; 5(1):34-42.
 

Keywords

Current Medical Mycology
Volume 7, Issue 2
June 2021
Pages 14-21

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History

  • Receive Date: 22 March 2021
  • Revise Date: 13 July 2021
  • Accept Date: 14 July 2021
  • First Publish Date: 14 July 2021

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