Antifungal properties of carvone and linalool against Malassezia species: Preliminary Screening Study

Document Type : Short Communication

Authors

1 Department of Medical Parasitology and Mycology,Shiraz University of Medical Sciences

2 Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

3 Pharmaceutical Sciences Research Center, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

4 Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

10.22034/cmm.2024.345248.1547

Abstract

Background and Purpose: By harnessing the power of nature, researchers can potentially discover new therapeutic options that are safe, effective, and sustainable for the management of diseases. Recently, natural products have been extensively studied for the treatment of diseases due to their diverse chemical composition and potential therapeutic properties. Therefore, this study aimed to investigate the antifungal activity of carvone and linalool against Malassezia species to find alternative treatments for pityriasis versicolor.
Materials and Methods: The in vitro antifungal activity of monoterpenes was assessed using a microdilution method, following the guidelines specified in the Clinical and Laboratory Standards Institute document M27-A3 with modifications, including the use of Christensen’s urea broth supplemented with various lipids to optimize the growth condition for Malassezia.
Results: The minimum inhibitory concentration ranges for linalool and carvone were found to be 0.3-5.4 and 0.3-24 mg/mL, respectively. Additionally, the growth of Malassezia species was inhibited at concentrations of 0.001-0.003 and 0.006-0.1 mg/mL for amphotericin B and ketoconazole, respectively.
Conclusion: Given the remarkable antifungal properties exhibited by linalool and carvone against Malassezia species, these terpene compounds have the potential to be utilized for the treatment of Malassezia infections, provided that additional research is conducted.

Keywords

Main Subjects

References

  1. Lorch JM, Palmer JM, Vanderwolf KJ, Schmidt KZ, Verant ML, Weller TJ, et al. Malassezia vespertilionis sp. nov.: a new cold-tolerant species of yeast isolated from bats. Persoonia. 2018;41(1):56-70.
  2. Lin SY, Lu PL, Tan BH, Chakrabarti A, Wu UI, Yang JH, et al. The epidemiology of non-Candida yeast isolated from blood: The Asia Surveillance Study. Mycoses. 2019;62(2):112-20.
  3. Zomorodian K, Mirhendi H, Tarazooie B, Zeraati H, Hallaji Z, Balighi K. Distribution of Malassezia species in patients with psoriasis and healthy individuals in Tehran, Iran. J Cutan Pathol. 2008;35(11):1027-31.
  4. Iraji A, Yazdanpanah S, Alizadeh F, Mirzamohammadi S, Ghasemi Y, Pakshir K, et al. Screening the antifungal activities of monoterpenes and their isomers against Candida species. J Appl Microbiol. 2020;129(6):1541-51.
  5. Silva KVS, Lima MIO, Cardoso GN, Santos AS, Silva GS, Pereira FO. Inibitory effects of linalool on fungal pathogenicity of clinical isolates of Microsporum canis and Microsporum gypseum. Mycoses. 2017;60(6):387-93.
  6. de Oliveira Lima MI, Araujo de Medeiros AC, Souza Silva KV, Cardoso GN, de Oliveira Lima E, de Oliveira Pereira F. Investigation of the antifungal potential of linalool against clinical isolates of fluconazole resistant Trichophyton rubrum. J Mycol Med. 2017;27(2):195-202.
  7. Nazzaro F, Fratianni F, Coppola R, Feo V. Essential Oils and Antifungal Activity. Pharmaceuticals (Basel). 2017;10(4):86.
  8. Santhanam J, Ghani A, Nadiah F, Basri DF. Antifungal activity of Jasminum sambac against Malassezia sp. and non-Malassezia sp. isolated from human skin samples. J Mycol. 2014;2014.
  9. Costa DC, Vermelho AB, Almeida CA, de Souza Dias EP, Cedrola SM, Arrigoni-Blank Mde F, et al. Inhibitory effect of linalool-rich essential oil from Lippia alba on the peptidase and keratinase activities of dermatophytes. J Enzyme Inhib Med Chem. 2014;29(1):12-7.
  10. Hsu CC, Lai WL, Chuang KC, Lee MH, Tsai YC. The inhibitory activity of linalool against the filamentous growth and biofilm formation in Candida albicans. Med Mycol. 2013;51(5):473-82.
  11. Rojas FD, Sosa Mde L, Fernandez MS, Cattana ME, Cordoba SB, Giusiano GE. Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method. Med Mycol. 2014;52(6):641-6.
  12. Velegraki A, Alexopoulos EC, Kritikou S, Gaitanis G. Use of fatty acid RPMI 1640 media for testing susceptibilities of eight Malassezia species to the new triazole posaconazole and to six established antifungal agents by a modified NCCLS M27-A2 microdilution method and Etest. J Clin Microbiol. 2004;42(8):3589-93.
  13. Iatta R, Figueredo LA, Montagna MT, Otranto D, Cafarchia C. In vitro antifungal susceptibility of Malassezia furfur from bloodstream infections. J Med Microbiol. 2014;63(Pt 11):1467-73.
  14. Carrillo‐Muñoz AJ, Rojas F, Tur‐Tur C, de Los Ángeles Sosa M, Diez GO, Espada CM, et al. In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species. Mycoses. 2013;56(5):571-5.
  15. Nakamura Y, Kano R, Murai T, Watanabe S, Hasegawa A. Susceptibility testing of Malassezia species using the urea broth microdilution method. Antimicrob Agents Chemother. 2000;44(8):2185-6.
  16. Rincon S, Cepero de Garcia MC, Espinel-Ingroff A. A modified Christensen's urea and CLSI broth microdilution method for testing susceptibilities of six Malassezia species to voriconazole, itraconazole, and ketoconazole. J Clin Microbiol. 2006;44(9):3429-31.
  17. Cafarchia C, Figueredo LA, Favuzzi V, Surico MR, Colao V, Iatta R, et al. Assessment of the antifungal susceptibility of Malassezia pachydermatis in various media using a CLSI protocol. Vet Microbiol. 2012;159(3-4):536-40.
  18. Yamada Y, Makimura K, Merhendi H, Ueda K, Nishiyama Y, Yamaguchi H, et al. Comparison of different methods for extraction of mitochondrial DNA from human pathogenic yeasts. Jpn J Infect Dis. 2002;55(4):122-5.
  19. Mirhendi H, Makimura K, Zomorodian K, Yamada T, Sugita T, Yamaguchi H. A simple PCR-RFLP method for identification and differentiation of 11 Malassezia species. J Microbiol Methods. 2005;61(2):281-4.
  20. Peano A, Pasquetti M, Tizzani P, Chiavassa E, Guillot J, Johnson E. Methodological Issues in Antifungal Susceptibility Testing of Malassezia pachydermatis. J Fungi (Basel). 2017;3(3):37.
  21. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999;12(4):564-82
  22. Herman A, Tambor K, Herman A. Linalool Affects the Antimicrobial Efficacy of Essential Oils. Curr Microbiol. 2016;72(2):165-72.
  23. Dias IJ, Trajano E, Castro RD, Ferreira GLS, Medeiros HCM, Gomes DQC. Antifungal activity of linalool in cases of Candida spp. isolated from individuals with oral candidiasis. Braz J Biol. 2018;78(2):368-74.

24.  Khan A, Ahmad A, Akhtar F, Yousuf S, Xess I, Khan LA, et al. Ocimum sanctum essential oil and its active principles exert their antifungal activity by disrupting ergosterol biosynthesis and membrane integrity. Res Microbiol. 2010;161(10):816-23.

  1. Aggarwal K, Khanuja S, Ahmad A, Santha Kumar T, Gupta VK, Kumar S. Antimicrobial activity profiles of the two enantiomers of limonene and carvone isolated from the oils of Mentha spicata and Anethum sowa. Flavour Fragr J. 2002;17(1):59-63.
  2. Moro IJ, Gondo GDGA, Pierri EG, Pietro RCLR, Soares CP, Sousa DPd, et al. Evaluation of antimicrobial, cytotoxic and chemopreventive activities of carvone and its derivatives. Braz J Pharm Sci. 2018;53.
  3. Rhimi W, Inyang Aneke C, Mosca A, Otranto D, Cafarchia C. In Vitro Azole and Amphotericin B Susceptibilities of Malassezia furfur from Bloodstream Infections Using E-Test and CLSI Broth Microdilution Methods. Antibiotics (Basel). 2020;9(6):361.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Current Medical Mycology
Volume 10, Continuous
2024
Pages 1-5

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History

  • Receive Date: 21 July 2024
  • Revise Date: 05 November 2024
  • Accept Date: 12 November 2024
  • Publish Date: 25 November 2024

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