Document Type : Original Articles
Authors
1
Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2
Department of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
3
Core Research Facilities (CRF), Isfahan University of Medical Sciences, Isfahan
4
Department of Medical Parasitology and Mycology, School of Medicine, Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
10.22034/cmm.2024.345245.1545
Abstract
Background and Purpose: Various efforts have been conducted to find potent and effective alternatives with natural origin and lesser side effects for the current antifungals. Here the antifungal effects of the Hydroalcoholic Extract (HE) and Lyophilized Extract (LE) of Prunus amygdalus hulls on clinical isolates of Candida albicans were determined and compared with the fluconazole antifungal.
Material and methods: Following the preparation of botanical compounds, the toxicity, cell viability, and high-performance liquid chromatography (HPLC) of phenolic compounds analyses were assayed. The broth microdilution method was applied to determine the minimum inhibitory concentration (MIC) values of fluconazole, LEs, and HEs against clinical isolates of C. albicans.
Results: According to the HPLC results, the HEs and LEs comprised the main nine components, of which chlorogenic and tannic acids were the most abundant compounds. The results of the toxicity assays revealed that no dilution of the extract was toxic to the cells, and the percentage of cell viability was similar to the control and above 90% in all dilutions. All isolates showed susceptibility to fluconazole (MIC range: 0.12 to 1 μg/mL). The MIC geometric mean (GM) of C. albicans isolates was 0.29 μg/mL, 11.47 μg/mL, and 48.50 μg/mL for fluconazole, LE, and HE, respectively.
Conclusion: Due to their insignificant side effects and cost-effectiveness, these extracts can be introduced as effective antifungals. Further, in-vivo studies and clinical trials should support the study results.
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