The effect of nanochitosans particles on Candida biofilm formation


1 Department of Medical Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

2 Department of Prosthodontics, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran


Background and Purpose: In people wearing dentures, the growth of various Candida species under the prosthesis
leads to the formation of biofilm, which can play the role of a reservoir for Candida and other kinds of microbes. Since
nano-chitosan particles can cause lasting antimicrobial activity, a more recent approach that utilizes acrylic resins with
nano-chitosan particles is proposed. Therefore, we aimed to study the inhibitory effect of nano-chitosan particles on the
biofilm formation of Candida species in acrylic resins.
Materials and Methods: In this analytical in-vitro study, acrylic resins with nano-chitosan particles with concentrations of
0, 1%, 5%, and 10% were put adjacent to the suspension of Candida cells isolated from the individuals’ mouth and biofilm
formation on resins was measured and compared. Finally, the data were analyzed using Kruskal-Wallis and Chi-square tests.
Results:The observed differences between unmodified acrylic resin (control) and acrylic resin with nano-chitosan
particles in terms of biofilm formation were significant (p <0.05), but no significant difference was found in the formation
of biofilm species on resins.
Conclusion: Biofilm formation of Candida species depends on acrylic resin type, in a way that by adding nano-chitosan
particles to acrylic resins, biofilm formation of Candida species was significantly reduced. To decrease the organization
of biofilm and denture stomatitis, the use of acrylics with nano-chitosan particles in producing dentures is recommended.


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Volume 2, Issue 2
June 2016
Pages 28-33
  • Receive Date: 09 July 2019
  • Revise Date: 08 October 2020
  • Accept Date: 09 July 2019
  • First Publish Date: 09 July 2019