Identification of clinical dermatophyte isolates obtained from Iran by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Authors

1 Mazandaran University of Medical Sciences

2 Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Bushehr University of Medical Sciences, Bushehr, Iran.

4 Babol University of Medical Sciences, Babol, Iran

5 Mazandaran University of Medical Sciences, Sari, Iran

6 Faculty of Medicine, University of Akdeniz, Antalya, Turkey.

7 aculty of Medicine, University of Akdeniz, Antalya, Turkey.

8 Department of Microbiology, Faculty of Medicine, University of Çukurova, Adana, Turkey

9 National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA

Abstract

Background and Purpose: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is widely used to discriminate among pathogenic microorganisms in clinical laboratories. The aim of this study was to assess the utility of MALDI-TOF MS in the routine identification of clinical dermatophyte isolates obtained from various geographical regions of Iran.

Materials and Methods: A total of 94 isolates, including Trichophyton interdigitale (n=44), T. rubrum (n=40), T. tonsurans (n=4), Microsporum canis (n=4), and Epidermophyton floccosum (n=1), were analyzed in this study. The identity of each isolate was determined by polymerase chani reaction amplification and sequencing of the internal transcribed spacer (ITS) region of nuclear-encoded ribosomal DNA and also MALDI-TOF MS. The obtained data by molecular approach were compared with MALDI-TOF MS.

Results: The MALDI-TOF MS led to the identification of 44 (47%) isolates at the species level by generating the spectral score values of ≥ 2.0. However, there was not sufficient agreement between the results of the molecular-based ITS identification methods and MALDI-TOF MS in the species identification of 16 (17%) isolates. The Bruker Daltonics database was also not able to identify protein spectra related to 12 isolates (13%), including T. interdigitale (n=5), T. rubrum (n=4), M. canis (n=2), and T. tonsurans (n=1).

Conclusion: According to the results, the utility of MALDI-TOF MS as a routine diagnostic tool for the accurate and reliable identification of dermatophytes can be justified whenever the protein spectra of a large set of worldwide clinical isolates are included in the commercial libraries. In addition, MALDI-TOF MS can be alternatively used to construct an in-house reference database.

Keywords

References

1. Hubka V, Peano A, Cmokova A, Guillot J. Common and emerging dermatophytoses in animals: well-known and new threats. In: Seyedmousavi S, de Hoog G, Guillot J, Verweij P, editors. Emerging and epizootic fungal infections in animals. Cham: Springer; 2018.
2. Drake LA, Dinehart SM, Farmer ER, Goltz RW, Graham GF, Hardinsky MK, et al. Guidelines of care for superficial mycotic infections of the skin: tinea corporis, tinea cruris, tinea faciei, tinea manuum, and tinea pedis. Guidelines/Outcomes Committee. American Academy of Dermatology. J Am Acad Dermatol. 1996; 34(2 Pt 1):282-6.
3. de Hoog GS, Dukik K, Monod M, Packeu A, Stubbe D, Hendrickx M, et al. Toward a novel multilocus phylogenetic taxonomy for the dermatophytes. Mycopathologia. 2017; 182(1-2):5-31.
4. Ansari S, Hedayati MT, Zomorodian K, Pakshir K, Badali H, Rafiei A, et al. Molecular characterization and in vitro antifungal susceptibility of 316 clinical isolates of dermatophytes in Iran. Mycopathologia. 2016; 181(1-2):89-95.
5. Hedayati MT, Armaki MT, Charati JY, Hedayati N, Seyedmousavi S, Denning DW. Burden of fungal infections in Iran. J Infect Dev Countr. 2018; 12(10):910-8.
6. Durdu M, Ilkit M, Tamadon Y, Tolooe A, Rafati H, Seyedmousavi S. Topical and systemic antifungals in dermatology practice. Expert Rev Clin Pharmacol. 2017; 10(2):225-37.
7. Zhan P, Dukik K, Li D, Sun J, Stielow JB, Gerrits van den Ende B, et al. Phylogeny of dermatophytes with genomic character evaluation of clinically distinct Trichophyton rubrum and T. violaceum. Stud Mycol. 2018; 89:153-75.
8. Jensen RH, Arendrup MC. Molecular diagnosis of dermatophyte infections. Curr Opin Infect Dis. 2012; 25(2):126-34.
9. L'Ollivier C, Cassagne C, Normand AC, Bouchara JP, Contet-Audonneau N, Hendrickx M, et al. A MALDI-TOF MS procedure for clinical dermatophyte species identification in the routine laboratory. Med Mycol. 2013; 51(7):713-20.
10. De Respinis S, Monnin V, Girard V, Welker M, Arsac M, Cellière B, et al. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry using the vitek ms system for rapid and accurate identification of dermatophytes on solid cultures. J Clin Microbiol. 2014; 52(12):4286-92.
11. Calderaro A, Motta F, Montecchini S, Gorrini C, Piccolo G, Piergianni M, et al. Identification of dermatophyte species after implementation of the in-house MALDI-TOF MS database. Int J Mol Sci. 2014; 15(9):16012-24.
12. Karabicak N, Karatuna O, Ilkit M, Akyar I. Evaluation of the bruker matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) system for the identification of clinically important dermatophyte species. Mycopathologia. 2015; 180(3-4):165-71.
13. Rezaei-Matehkolaei A, Makimura K, de Hoog S, Shidfar MR, Zaini F, Eshraghian M, et al. Molecular epidemiology of dermatophytosis in Tehran, Iran, a clinical and microbial survey. Med Mycol. 2013; 51(2):203-7.
14. Ahmadi B, Mirhendi H, Makimura K, de Hoog GS, Shidfar MR, Nouripour-Sisakht S, et al. Phylogenetic analysis of dermatophyte species using DNA sequence polymorphism in calmodulin gene. Sabouraudia. 2016; 54(5):500-14.
15. Sanguinetti M, Posteraro B. Identification of molds by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2017; 55(2):369-79.
16. Sanguinetti M, Posteraro B. MALDI-TOF mass spectrometry: any use for Aspergilli? Mycopathologia. 2014; 178(5-6):417-26.
17. Posteraro B, De Carolis E, Vella A, Sanguinetti M. MALDI-TOF mass spectrometry in the clinical mycology laboratory:
identification of fungi and beyond. Expert Rev Proteomics. 2013; 10(2):151-64.
18. Karabıçak N, Karatuna O, İlkit M, Akyar I. Evaluation of the Bruker matrix-assisted laser desorption–ionization time-of-flight mass spectrometry (MALDI-TOF MS) system for the identification of clinically important dermatophyte species. Mycopathologia. 2015; 180(3-4):165-71.
19. Theel ES, Hall L, Mandrekar J, Wengenack NL. Dermatophyte identification using matrix-assisted laser desorption ionization–time of flight mass spectrometry. J Clin Microbiol. 2011; 49(12):4067-71.
20. L’Ollivier C, Ranque S. MALDI-TOF-based dermatophyte identification. Mycopathologia. 2017; 182(1-2):183-92.
21. L’Ollivier C, Cassagne C, Normand AC, Bouchara JP, Contet-Audonneau N, Hendrickx M, et al. A MALDI-TOF MS procedure for clinical dermatophyte species identification in the routine laboratory. Med Mycol. 2013; 51(7):713-20.
22. Packeu A, De Bel A, l'Ollivier C, Ranque S, Detandt M, Hendrickx M. Fast and accurate identification of dermatophytes by matrix-assisted laser desorption ionization–time of flight mass spectrometry: validation in the clinical laboratory. J Clin Microbiol. 2014; 52(9):3440-3.
23. Alshawa K, Beretti JL, Lacroix C, Feuilhade M, Dauphin B, Quesne G, et al. Successful identification of clinical dermatophyte and Neoscytalidium species by matrix-assisted laser desorption ionization–time of flight mass spectrometry. J Clin Microbiol. 2012; 50(7):2277-81.
24. Erhard M, Hipler UC, Burmester A, Brakhage AA, Wöstemeyer J. Identification of dermatophyte species causing onychomycosis and tinea pedis by MALDI‐TOF mass spectrometry. Exp Dermatol. 2008; 17(4):356-61.
25. Nenoff P, Erhard M, Simon JC, Muylowa GK, Herrmann J, Rataj W, et al. MALDI-TOF mass spectrometry–a rapid method for the identification of dermatophyte species. Med Mycol. 2013; 51(1):17-24.
26. Abastabar M, Rezaei-Matehkolaei A, Shidfar MR, Kordbacheh P, Mohammadi R, Shokoohi T, et al. A molecular epidemiological survey of clinically important dermatophytes in Iran based on specific RFLP profiles of beta-tubulin gene. Iran J Public Health. 2013; 42(9):1049-57.
27. Rezaei-Matehkolaei A, Rafiei A, Makimura K, Gräser Y, Gharghani M, Sadeghi-Nejad B. Epidemiological aspects of dermatophytosis in Khuzestan, southwestern Iran, an update. Mycopathologia. 2016; 181(7-8):547-53.
28. Ahmadi B, Mirhendi H, Shidfar MR, Nouripour-Sisakht S, Jalalizand N, Geramishoar M, et al. A comparative study on morphological versus molecular identification of dermatophyte isolates. J Mycol Med. 2015; 25(1):29-35.
29. Gräser Y, Scott J, Summerbell R. The new species concept in dermatophytes-a polyphasic approach. Mycopathologia. 2008; 166(5-6):239.
Current Medical Mycology
Volume 5, Issue 2
June 2019
Pages 22-26

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History

  • Receive Date: 09 July 2019
  • Revise Date: 07 September 2020
  • Accept Date: 09 July 2019
  • Publish Date: 01 June 2019

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