Antifungal effect of the effect of Securigera securidaca L. vaginal gel on Candida species

Document Type : Original Articles

Authors

1 Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

2 Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

3 Department of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

4 Student Research Committee, School of Medicine, Bam University of Medical Sciences, Bam, Iran

Abstract

Background and Purpose: Candida species are opportunistic fungi, capable of causing acute and chronic infections in the gastrointestinal tract, vagina, and oral mucosa, among which Candida albicans is the most important species. The Securigera securidaca L. is used as an antiseptic to treat some diseases in traditional Iranian medicine. The aim of this study was to evaluate the antimicrobial activity of S. securidaca extracts and vaginal gel against different Candida species.
Materials and Methods: Antifungal effects of different extracts and vaginal gel of S. securidaca were investigated against Candida species. By using well diffusion test, different concentrations of the collected S. securidaca extracts and vaginal gel were examined to test their antifungal activity against C. albicans, C. parapsilosis, and C. krusei.
Results: The ethanol extract and vaginal gel with the ethanol extract of S. securidaca showed the most anti-fungal activity against all three strains.
Conclusion: The S. securidaca extract had a significant inhibitory effect on the different species of Candida; however, the highest inhibitory effect was found against C. albicans. In order to treat candidiasis, more research is required to check the efficacy of this plant in this domain.



Keywords

References

1. Ramesh P, Okigbo R. Effects of plants and medicinal plant combinations as anti-infectives. Afr J Pharm Pharmacol. 2008; 2(7):130-5.
2. Hashemi S, Asgarpanah J, Alaee Z, Sadeghian S, Hasani H, Azimi A. In vitro antifungal activity of four medicinal plants used in Iranian Traditional Medicine. Res J Pharmacogn. 2014; 1(1):39-43.
3. Anaissie EJ, McGinnis MR, Pfaller MA. Clinical mycology. Ann Internal Med. 2003; 138(9):776.
4. Rios JL, Recio MC. Medicinal plants and antimicrobial activity. J Ethnopharmacol. 2005; 100(1-2):80-4.
5. Arif T, Bhosale J, Kumar N, Mandal T, Bendre R, Lavekar G, et al. Natural products–antifungal agents derived from plants. J Asian Natural Prod Res. 2009; 11(7):621-38.
6. Jamshidzadeh A, Pasdaran A, Heidari R, Hamedi A. Pharmacognostic and anti-inflammatory properties of Securigera securidaca seeds and seed oil. Res J Pharmacogn. 2018; 5(3): 31-9.
7. Tofighi Z, Sabzevari O, Rezaei Taleqani Z, Yassa N. Investigation of securigera securidaca seeds extract and different fractions on serum glucose, blood factors and liver morphology in diabetic animals. Iran J Endocrinol Metab. 2016; 18(1):37-45.
8. Mard S, Bahari Z, Eshaghi N, Farbood Y. Antiulcerogenic effect of Securigera securidaca L. seed extract on various experimental gastric ulcer models in rats. Pak J Biol Sci. 2008; 11(23):2619.
9. Hajzadeh M, Rajaei Z, Ghamami G, Tamiz A. The effect of Salvia officinalis leaf extract on blood glucose in streptozotocin-diabetic rats. Pharmacologyonline. 2011; 1:213-20.
10. Lan YB, Huang YZ, Qu F, Li JQ, Ma LJ, Yan J, et al. Time course of global gene expression alterations in Candida albicans during infection of HeLa cells. Bosn J Basic Med Sci. 2017; 17(2):120-31.
11. Shirani M, Samimi A, Kalantari H, Madani M, Zanganeh AK. Chemical composition and antifungal effect of hydroalcoholic extract of Allium tripedale (Tvautv.) against Candida species. Curr Med Mycol. 2017; 3(1):6-12.
12. Price MF, LaRocco MT, Gentry LO. Fluconazole susceptibilities of Candida species and distribution of species recovered from blood cultures over a 5-year period. Antimicrob Agents Chemother. 1994; 38(6):1422-4.
13. Nasrollahi Z, Yadegari MH, Roudber Mohammadi S, Roudbary M, Poor MH, Nikoomanesh F, et al. Fluconazole resistance Candida albicans in females with recurrent Vaginitis and Pir1 overexpression. Jundishapur J Microbiol. 2015; 8(9):e21468.
14. Richter SS, Galask RP, Messer SA, Hollis RJ, Diekema DJ, Pfaller MA. Antifungal susceptibilities of Candida species causing vulvovaginitis and epidemiology of recurrent cases. J Clin Microbiol. 2005; 43(5):2155-62.
15. Wasser SP. Medicinal mushroom science: history, current status, future trends, and unsolved problems. Int J Med Mushrooms. 2010; 12(1):281-3.
16. Das K, Tiwari R, Shrivastava D. Techniques for evaluation of medicinal plant products as antimicrobial agent: current methods and future trends. J Med Plants Res. 2010; 4(2):104-11.
17. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal. 2016; 6(2):71-9.
18. Kim HJ, Suh HJ, Lee CH, Kim JH, Kang SC, Park S, et al. Antifungal activity of glyceollins isolated from soybean elicited with Aspergillus sojae. J Agricd Food Chemi. 2010; 58(17):9483-7.
19. Subcommittee on Antifungal Susceptibility Testing of the ESCMID European Committee for Antimicrobial Susceptibility Testing. EUCAST Technical Note on the method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for conidia-forming moulds. Clin Microbiol Infect. 2008; 14(10):982-4.
20. Cavalcanti Filho JR, Silva TF, Nobre WQ, Oliveira de Souza LI, Silva e Silva Figueiredo CS, Figueiredo RC, et al. Antimicrobial activity of Buchenavia tetraphylla against Candida albicans strains isolated from vaginal secretions. Pharm Biol. 2017; 55(1):1521-7.
21. Rehman A, Rehman A, Ahmad I. Antibacterial, antifungal, and insecticidal potentials of Oxalis corniculata and its isolated compounds. Int J Anal Chem. 2015; 2015:842468.
22. Pfaller M, Diekema D. Epidemiology of invasive candidiasis: a persistent public health problem. Clin Microbiol Rev. 2007; 20(1):133-63.
23. Calderone RA, Clancy CJ. Candida and candidiasis. Washington, D.C: American Society for Microbiology Press; 2011.
24. Pfaller MA, Houston A, Coffmann S. Application of CHROMagar Candida for rapid screening of clinical specimens for Candida albicans, Candida tropicalis, Candida krusei, and Candida (Torulopsis) glabrata. J Clin Microbiol. 1996; 34(1):58-61.
25. Beighton D, Ludford R, Clark DT, Brailsford SR, Pankhurst CL, Tinsley GF, et al. Use of CHROMagar Candida medium for isolation of yeasts from dental samples. J Clin Microbiol. 1995; 33(11):3025-7.
26. Kirkpatrick WR, Turner TM, Fothergill AW, McCarthy DI, Redding SW, Rinaldi MG, et al. Fluconazole disk diffusion susceptibility testing of Candida species. J Clin Microbiol. 1998; 36(11):3429-32.
27. Behbahani M, Shanehsazzadeh M, Shokoohinia Y, Soltani M.
Evaluation of anti-herpetic activity of methanol seed extract and fractions of securigera securidaca in vitro. J Antivir Antiretrovir. 2013; 5(4):72-6.
28. Sadat-Ebrahimi S, Hassanpoor Mir M, Amin G, Hajimehdipoor H. Identification of amino acids in Securigera securidaca, a popular medicinal herb in Iranian folk medicine. Res J Pharmacog. 2014; 1(1):23-6.
29. Ibrahim RM, El-Halawany AM, Saleh DO, El Naggar EM, El-Shabrawy AE, El-Hawary SS. HPLC-DAD-MS/MS profiling of phenolics from Securigera securidaca flowers and its anti-hyperglycemic and anti-hyperlipidemic activities. Rev Bras Farmacog. 2015; 25(2):134-41.
30. Yarmolinsky L, Huleihel M, Zaccai M, Ben-Shabat S. Potent antiviral flavone glycosides from Ficus benjamina leaves. Fitoterapia. 2012; 83(2):362-7.
31. Kim TH, Ku SK, Lee IC, Bae JS. Anti-inflammatory effects of kaempferol-3-O-sophoroside in human endothelial cells. Inflamm Res. 2012; 61(3):217-24.
32. Tofighi Z, Asgharian P, Goodarzi S, Hadjiakhoondi A, Ostad SN, Yassa N. Potent cytotoxic flavonoids from Iranian Securigera securidaca. Med Chem Res. 2014; 23(4):1718-24.
33. Cushnie TT, Lamb AJ. Recent advances in understanding the antibacterial properties of flavonoids. Int J Antimicrob Agents. 2011; 38(2):99-107.
34. Lim YH, Kim IH, Seo JJ. In vitro activity of kaempferol isolated from the Impatiens balsamina alone and in combination with erythromycin or clindamycin against Propionibacterium acnes. J Microbiol. 2007; 45(5):473-7.
35. Basile A, Giordano S, López-Sáez JA, Cobianchi RC. Antibacterial activity of pure flavonoids isolated from mosses. Phytochemistry. 1999; 52(8):1479-82.
36. Sato Y, Suzaki S, Nishikawa T, Kihara M, Shibata H, Higuti T. Phytochemical flavones isolated from Scutellaria barbata and antibacterial activity against methicillin-resistant Staphylococcus aureus. J Ethnopharmacol. 2000; 72(3):483-8.
37. Cushnie TT, Hamilton VE, Lamb AJ. Assessment of the antibacterial activity of selected flavonoids and consideration of discrepancies between previous reports. Microbiol Res. 2003; 158(4):281-9.
38. Abbassy MA, Kadous EA, Abd-Allah E-SA, Marei GI. Isolation and identification of cardenolide compounds of gomphocarpus sinaicus and their fungicidal activity against soil borne and post harvest fungi. J Life Sci. 2012; 6(9):985.
Current Medical Mycology
Volume 5, Issue 3
September 2019
Pages 31-35

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History

  • Receive Date: 20 May 2019
  • Revise Date: 15 July 2019
  • Accept Date: 28 August 2019
  • First Publish Date: 01 September 2019

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